Time course for antioxidants production by Antrodia camphorata in submerged culture

深層培養中樟芝抗氧化物產生之時程

毛正倫(Jeng-Leun Mau) ； 黃珮甯(Pei-Ning Huang) ； 黃仕政(Shih-Jeng Huang) ； 陳勁初(Chin-Chu Chen)

《Fungal Science》 18卷3&4期 (2003/12) Pp. 59-71

https://doi.org/10.7099/FS.200312.0059

摘要

The mycelia of Antrodia camphorata (Chang & Chou) Wu, Ryvarden & Chang were grown in a 50-ton fermentor for 18 days. The antioxidant properties and antioxidant components of methanolic extracts from mycelia at different days of incubation were studied. In the antioxidant activity by the conjugated diene method, EC50 value of mycelia at day 18 (1.46 mg/mL) was the best, followed by values of mycelia at days 10, 13 and 16. Reducing powers were good (＞ 0.64) at concentrations of methanolic extracts higher than 2.5 mg/mL and EC50 values were considerately low (0.40–0.53 mg/mL) for mycelia at days 7 to 16. At 0.5 mg/mL, the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radicals was excellent (93.2.93.9%) and EC50 values were extremely low (0.27 mg/mL) for mycelia at days 7 to 16. The chelating effects on ferrous ions were good (58.9.92.3%) at 2.5 to 10 mg/mL. Contents of naturally occurring antioxidant components were found in the order of total phenols ＞ tocopherols ＞ ascorbic acid ＞ β-carotene. Methanolic extracts from A. camphorata red mycelia in submerged culture were good in the antioxidant properties tested, except for the scavenging effect on hydroxyl ions. More specifically, the antioxidant properties were good for mycelia at days 10 to 16.

關鍵字

抗氧化力 ； 抗氧化成份 ； 清除作用 ； 樟芝 ； 螯合作用 ； 還原力

並列摘要

The mycelia of Antrodia camphorata (Chang & Chou) Wu, Ryvarden & Chang were grown in a 50-ton fermentor for 18 days. The antioxidant properties and antioxidant components of methanolic extracts from mycelia at different days of incubation were studied. In the antioxidant activity by the conjugated diene method, EC50 value of mycelia at day 18 (1.46 mg/mL) was the best, followed by values of mycelia at days 10, 13 and 16. Reducing powers were good (＞ 0.64) at concentrations of methanolic extracts higher than 2.5 mg/mL and EC50 values were considerately low (0.40–0.53 mg/mL) for mycelia at days 7 to 16. At 0.5 mg/mL, the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radicals was excellent (93.2.93.9%) and EC50 values were extremely low (0.27 mg/mL) for mycelia at days 7 to 16. The chelating effects on ferrous ions were good (58.9.92.3%) at 2.5 to 10 mg/mL. Contents of naturally occurring antioxidant components were found in the order of total phenols ＞ tocopherols ＞ ascorbic acid ＞ β-carotene. Methanolic extracts from A. camphorata red mycelia in submerged culture were good in the antioxidant properties tested, except for the scavenging effect on hydroxyl ions. More specifically, the antioxidant properties were good for mycelia at days 10 to 16.

並列關鍵字

antioxidant activity ； antioxidant components ； Antrodia camphorata ； chelating effect ； reducing