When the Italian Renaissance reached its late and fading stage, a school of artists from 1520 to about 1600 devoted their own skills and mind streams for presenting their personality and creativity in art. Their creativity derailed the classic doctrine. Eventually, their creation became a fashion that possessed a unique artistic style, which is different from the old establishment advocated by their predecessors. Although this fashion prevailed in the period, Mannerism was often labeled as a recession of the Renaissance by the late critics. With their versatile innovations, this school of artists pioneered a new style of creative art, and furthermore, motivated later artists to apply multidimensional thoughts into their creativity. This article is motivated by the similarities between the evolution of Mannerism in the 16th century and the evolution of art since the 20th century. This article starts with a search for the root of Mannerism, followed by clarification for unsettled problems in order to establish the historical stature for Mannerism.