Title

細菌之核苷核糖化毒素:綠膿桿菌外毒素A

Translated Titles

ADP-Ribosylating Bacterial Toxins: Pseudomonas Exotoxin A

Authors

陳作琳(Tso-Ling Chen);林立中(Lee-Chung Lin);黃昭蓮(Jau-Lang Hwang);林嘉伯(Chia-Po Lin)

Key Words

核苷核糖化 ; 細菌毒素 ; 綠膿桿菌外毒素A ; ADP-ribosylation ; bacterial toxins ; Pseudomonas exotoxin A PEA

PublicationName

藥物食品分析

Volume or Term/Year and Month of Publication

4卷2期(1996 / 06 / 01)

Page #

107 - 114

Content Language

繁體中文

Chinese Abstract

對於一些細菌所產生的毒素,經由作用於眞核細胞內某些重要蛋白質的核苷核糖化反應(ADP-ribosylating reaction)而影響其正常的生理功能已有大致的了解。大部份這類的毒素主要是由兩個部份(moieties),A和B所組成。B部份負責其分子本身與毒性敏感細胞表面特殊受器的結合,而A部份則負責執行核苷核糖化(ADP-ribosylate)細胞內蛋白質的酵素活性。綠膿桿菌外毒素A (Pseudomonas、exotoxin A,簡稱PEA),是綠膿桿菌所產生的細胞外產物中最具毒性的成份。此一外泌性蛋白質毒素主要由三個區域(domains)所組成:即區域Ⅰ,區域Ⅱ和區域Ⅲ,其主要功能分別與細胞的結合(binding)、在細胞內的移位(translocation)和發揮核苷核糖化活性(ADP-ribosylating activity)有關。吾人若應用綠膿桿菌外毒素A之核苷核糖化反應毒殺細胞的功能,一般皆相信可以作為製備免疫毒素(immunotoxins)的最佳選譯。在本篇綜論中,吾人將討論核苷核糖化細菌毒素(ADP-ribosylating bacterial toxin)的結構、作用機制,並對於綠膿桿菌外毒素A,以及綠膿桿菌外毒素A和其他核苷核糖化毒素(ADP-ribosylating toxin)的異同作一簡介。

English Abstract

It is well known that a number of toxins produced by bacteria exert their action by ADP-ribosylating reaction to certain proteins which are essential for normal eukaryotic cellular functions. Most of these toxins are composed of two moieties, A and B. The B moiety mediates the binding to the specific receptor on the surface of toxin-sensitive cells, while the A moiety is responsible for the enzymatic ADP-ribosylating activity. Pseudomonas exotoxin A (PEA) is the most toxic component of the extracellular prod ucts produced by Pseudomonas aeruginosa. The three domain model of PEA has been well established domain Ⅰ, domain Ⅱ, and domain Ⅲ exerting binding, translocation, and ADP-ribosylating activities, respectively. Because of the cytotoxic ADP-ribosylating nature of PEA, it has been suggested as a good candidate in the preparation of immunotoxins. In this minireview article, we discuss the structure and function of the bacterial ADP-ribosylating toxins including PEA and compare the differences particularly between PEA and other valevant toxins.

Topic Category 醫藥衛生 > 藥理醫學