Title

Antioxidative Effect of Lactic Acid Bacteria: Intact Cells vs. Intracellular Extracts

Translated Titles

乳酸菌之抗氧化作用:菌體與胞內物抗氧化能力之比較

Authors

歐珠琴(Chu-Chyn Ou);呂聰明(Tsong-Ming Lu);蔡肇基(Jaw-Ji Tsai);顏志恒(Jyh-Herng Yen);陳暉雯(Haw-Wen Chen);林美吟(Meei-Yn Lin)

Key Words

乳酸菌 ; 抗氧化作用 ; 低密度脂蛋白氧化作用 ; 菌體 ; 胞內物 ; lactic acid bacteria ; antioxidation ; LDL oxidation ; intact cells ; intracellular extracts

PublicationName

藥物食品分析

Volume or Term/Year and Month of Publication

17卷3期(2009 / 06 / 01)

Page #

209 - 216

Content Language

英文

Chinese Abstract

本研究比較兩株乳酸菌Bifidobacterium longum 和Lactobacillus delbrueckiisubsp. bulgaricus其菌體及胞內物的抗氧化能力。結果顯示,B. longum 和L.delbrueckii subsp. bulgaricus 在10^9 CFU/mL時,菌體及胞內物具有清除DPPH自由基能力(70.4-75.1%)、抑制liposome 過氧化(25-31%)以及顯著降低Intestine 407腸細胞脂質過氧化物(malondialdehyde, MDA)的生成。此外,本研究再進一步利用這兩株具有抗氧化能力乳酸菌的活菌體及其胞內物,探討對腦中風病人(cerebrovascular accident, CVA)和健康受試者血液中低密度脂蛋白(LDL)氧化作用的影響。LDL的氧化作用是以監測LDL conjugateddienes 形成的起始時間(lag time)爲指標。當B. longum和L. delbrueckii subsp. bulgaricus 以10^9 cfu/mL的菌數分別處理LDL時,LDL氧化作用的起始時間顯著被延長,健康受試者血液中LDL氧化被抑制效果優於CVA病人,而當以B. longum和L. delbrueckii subsp. bulgaricus 10^9 CFU/mL 菌數的胞內物處理CVA病人及健康受試者的LDL時,LDL氧化作用的起始時間顯著被延長超過180分鐘,此結果表示,抑制LDL氧化作用以乳酸菌的胞內物優於菌體。推論乳酸菌的胞內物具有效的抑制因子。

English Abstract

The present study compared the anti-oxidative ability of intact cells and intracellular extracts of two lactic acid bacterial strains, Bifidobacterium longum and Lactobacillus delbrueckii ssp. bulgaricus. Results showed that both intact cells and intracellular extracts of 10^9 cells of B. longum and L. delbrueckii ssp. bulgaricus had the ability to scavengeα-diphenyl-β-picrylhydrazyl (DPPH) free radical by 70.4-75.1%, to inhibit liposome peroxidation by 25-31%, and to decrease significantly the malondialdehyde (MDA) production in Intestine 407 cells. The effect of intact cells and intracellular extracts of these two bacterial strains on the oxidation of low density lipoprotein (LDL) isolated from cerebrovascular accident (CVA) patients and healthy subjects was also compared. Oxidation of LDL was monitored by measuring the lag time for the formation of conjugated dienes in isolated LDL particles. When LDL was treated respectively with 109 intact cells of B. longum and L. delbrueckii ssp. bulgaricus, the lag time of oxidation of LDL was prolonged significantly. The extent of inhibition was greater on LDL isolated from healthy subjects than from CVA patients. When LDL from either CVA patients or healthy subjects was treated with intracellular extracts of 10^9 cells of B. longum and L. delbrueckii ssp. bulgaricus, respectively, the copper-mediated oxidation was extensively inhibited with a lag time exceeding 180 min. Results from this study show a greater inhibitory effect on LDL oxidation exerted by the intracellular extract than the intact cells, suggesting the presence of effective inhibitory factors in the intracellular extract.

Topic Category 醫藥衛生 > 藥理醫學