Burkholderia gladioli引起之鳳梨果腐病

許秀惠(S. H. Hseu) ； 曾偉凡(W. F. Zeng) ； 賴婉綺(W. C. Lai) ； 潘雅碧(Y. P Pan) ； 林俊義(Y. C. Lin)

《植物病理學會刊》 17卷2期 (2008/06) Pp. 157-167

https://doi.org/10.6649/PPB.200806_17(2).0006

摘要

將採集自南投縣及嘉義縣之無病徵鳳梨剖開後發現花室組織褐化及輕微軟腐的現象。經柯霍氏法則確認分離自鳳梨之病原爲細菌；生理生化測定顯示病原細菌爲革蘭氏陰性，桿狀，具單極生一至多根鞭毛之好氣菌，在KB培養基上不會產生螢光色素，在NA上爲白色菌落，培養後產生可溶性黃色色素，此結果顯示該病原細菌與Burkholderia gladioli的特性相近，經Biolog鑑定以及脂肪酸分析結果亦爲B. gladioli。對B. gladioli具專一性之CMG-23-1/G-23-2引子組對病原菌進行PCR鑑定，可產生388 bp大小的專一性DNA條帶，再以f8-27/r1510引子對增幅該病原菌之16S rDNA片段進行定序與鑑定，確認該病原菌爲B. gladioli。由B. gladioli引起之鳳梨果腐病在鳳梨病害上爲首次記載。以11種殺菌劑測試在一般使用濃度下對該病菌生長之抑制效果，顯示供試11種藥劑包括歐索林酸、鋅錳乃浦、四環黴素、氫氧化銅、嘉賜黴素、嘉賜銅、鏈四環黴素、多保鏈黴素、鏈黴素、鹼性氯氧化銅及三元硫酸銅等藥劑均能抑制病原菌生長，且以歐索林酸抑制效果最佳。

關鍵字

鳳梨 ； 果腐病 ； 聚合酶連鎖反應

並列摘要

A new record of pineapple disease its Taiwan was investigated. The disease did not cause apparent symptoms on pineapple plants or fruit surfaces, but it induced browning and mild soft-rot to the fruit flesh, similar to the pineapple marbling disease caused by Pantoea ananatis. Based on the symptom and pathological studies, physiological and biochemical analyses, Biolog GN2 and MIDI Sherlock Microbial Identification comparisons, the pathogen was identified as Burkholderia gladioli. PCR identification showed that the pathogen, same with B. gladioli isolated from various host plants, contained a DNA segment of 388 bp that is not found in P. ananatis. Results from 16S rDNA sequencing and comparison revealed that the degree of similarity to the ones of B. gladioli reached 99%. Besides, eleven commercial agrochemicals were tested and all found to inhibit the growth of this pathogen; among which, oxolinic acid was identified to be the most effective one.

並列關鍵字

pineapple ； fruit-rot disease ； PCR ； Burkhoderia gladioli