應用ISSR解析山木麻黃國際種原之遺傳變異與種原關係

Assessment of Genetic Variation and Relationships of International Provenances of Casuarina junghuhniana Using ISSR

何坤益(Kuen-Yih Ho) ； 楊政川(Jenq-Chuan Yang) ； 鄧書麟(Shu-Lin Deng) ； 陳財輝(Tsai-Huei Chen)

《台灣林業科學》 19卷1期 (2004/03) Pp. 79-88

https://doi.org/10.7075/TJFS.200403.0079

摘要

Genetic variation and population structure of 113 individuals sampled from 13 provenances of Casuarina junghuhniana Miq. were examined using inter simple sequence repeat (ISSR) fingerprinting. The 8 ISSR primers assayed yielded 52 polymorphic bands. Analysis of molecular variance (AMOVA) revealed that the variance component between native and cultivated provenance groups was 14.31% (p＜0.01), among provenances within groups was 10.33% (p＜0.001), and among individuals within provenances was 75.36% (p＜0.001). Remarkable genetic differentiation and low levels of gene flow were detected between native provenances due to geographical isolation. In contrast, cultivated provenances had low genetic differentiation perhaps due to coancestry. The UPGMA analysis revealed that the cultivated provenances from Kenya and Tanzania might have originated from native provenances from East Java, Lombok, Bali, and Sumbawa. The native provenances from Timor revealed geographic relationships among these islands.

關鍵字

山木麻黃 ； 基因流傳 ； 遺傳分化 ； 簡單序列重複 ； 分子變方分析

並列摘要

Genetic variation and population structure of 113 individuals sampled from 13 provenances of Casuarina junghuhniana Miq. were examined using inter simple sequence repeat (ISSR) fingerprinting. The 8 ISSR primers assayed yielded 52 polymorphic bands. Analysis of molecular variance (AMOVA) revealed that the variance component between native and cultivated provenance groups was 14.31% (p＜0.01), among provenances within groups was 10.33% (p＜0.001), and among individuals within provenances was 75.36% (p＜0.001). Remarkable genetic differentiation and low levels of gene flow were detected between native provenances due to geographical isolation. In contrast, cultivated provenances had low genetic differentiation perhaps due to coancestry. The UPGMA analysis revealed that the cultivated provenances from Kenya and Tanzania might have originated from native provenances from East Java, Lombok, Bali, and Sumbawa. The native provenances from Timor revealed geographic relationships among these islands.

並列關鍵字

Casuarina junghuhniana ； gene flow ； genetic differentiation ； ISSR ； AMOVA