Beneficial effects of fasting regimens on periodontal tissues in experimental periodontitis mice model
Lia Kartika Wulansari；Boosana Kaboosaya1, Masud Khan；Mariko Takahashi；Hidemi Nakata；Shinji Kuroda；KazuhiroAoki；ShoheiKasugai
Periodontitis ； Intermittent Fasting ； Prolonged Fasting ； Animal Experiment
Journal of International Dental and Medical Research
|Volume or Term/Year and Month of Publication||
11卷2期（2018 / 07 / 01）
362 - 369
Previous studies demonstrated that fasting has beneficial effects on overall health, extending lifespan and that it rejuvenates cells, promoting tissue regeneration. Periodontitis is a prevalent oral inflammatory disease that leads to alveolar bone loss and linked to several systemic health problems. Ligature-induced periodontitis model is considered to be similar to human periodontitis in various aspect and is one of the most used animal models in periodontal research. The present study aims to investigate the effect of fasting regimen in experimentally-induced periodontitis in mice. In this study we applied two different kinds of fasting regimens: Intermittent fasting (IF / every other day fasting)and prolonged fasting (PF / 2 days fasting and 5 days free food intake) for 4 weeks. We found that both fasting regimen groups showed less amount of bone loss compared to the nonfasting groups at the ligature-periodontitis site, furthermore also at the contralateral side of the maxillae where the physiological bone loss occurs. PQCT and calcein-labeled histomorphometric analyses showed higher bone regeneration capacity in the fasting groups than in the nonfasting groups. Bone marrow cells from the femurs of the fasting groups produced more mineralized modules than the nonfasting groups. Data from this study showed fasting would be beneficial for resisting bone destruction in periodontitis and for maintaining periodontal bone health, which could be due to the increase of osteoprogenitor cells in bone marrow.