Effect of Aquilaria crassna crude extract on osteogenic activity of MC3T3-E1 cells

Yosnarong Sirimethawong ； Rungarun Kriangkrai ； Paiboon Jitprasertwong ； Thidarat Angwarawong ； Boontharika Chuenjitkuntaworn

《Journal of International Dental and Medical Research》 11卷2期 (2018/07) Pp. 510-519

摘要

The present study aimed to investigate the effect of the Aquilaria crassna extract (AE) on osteogenic activity including cell viability, cell proliferation, cell attachment and osteogenic differentiation of osteoblast like cells (MC3T3-E1). These were evaluated in term of cell viability, cell proliferation and cell attachment by MTT assays. While the methods of alkaline phosphatase (ALP) staining and activity kits, quantitative real-time PCR of osteogenic gene expression and Alizarin Red-S staining were performed to evaluate the effect of the AE on osteogenic differentiation. The results showed that the concentration of AE at 10, 25 and 50 μg/ml had no cytotoxicity. The AE (50 μg/ml) effectively enhanced cell proliferation at 24 h and increased cell attachment at 4 h and 24 h in MC3T3-E1 cells. The AE (50 μg/ml) effectively promoted osteogenic differentiation of MC3T3-E1 cells by increasing an ALP activity, an expression of osteogenic gene markers (collagen type I, ALP, bone sialoprotein and osteocalcin) and a mineral deposition. In conclusion, the data presented in this study showed a potential of Aquilaria crassna extract to improve initial cell attachment and proliferation, and to stimulate osteogenic differentiation in MC3T3-E1 cells.

關鍵字

Aquilaria ； extract ； osteogenic ； osteoblast