Biodegradation and Compressive Strength Test of Scaffold with Different Ratio as Bone Tissue Engineering Biomaterial

Prasiddha Mahardhika El Fadhlallah ； Anita Yuliati ； Pratiwi Soesilawati ； Pradipta Pitaloka

《Journal of International Dental and Medical Research》 11卷2期 (2018/07) Pp. 587-590

摘要

Bone destruction in oral cavity due to trauma, chronic infection, congenital malformations, or surgical procedure have most often been treated using autologous bone-grafting procedure. Another solution emerged in tissue engineering is the use of scaffold. Calcium carbonate is one of potential ceramic materials that have osteoconductive properties that can be used as scaffold constituents. To investigate the comparative bio-degradation value and compressive strength of chitosan, gelatin, and calcium carbonate scaffold with various ratio. The scaffold were made using freeze-drying method. Degradation test was done by dissolving scaffold in PBS containing 1.6 μg/ml of the lysozyme. Compressive strength test is done by using autograft tool with load cell compress machine 100 kN. Degradation rate of scaffolds with ratio 40:60 was lower than scaffolds with 30:70 ratio. Compressive strength of scaffold with ratio 40:60 was higher than 30:70 ratio scaffold. The data were analyzed using T-test and showed significant difference in degradation rate and compressive strength test. The scaffolds with ratio 40:60 have better bio-degradation rate and compressive strength properties than scaffolds with ratio 30:70. The chitosan, gelatin and calcium carbonate scaffolds with ratio 40:60 have potency as an alternative biomaterial in bone tissue engineering.

關鍵字

Tissue engineering ； Bone ； Scaffold ； Biodegradation ； Compressive strength