Seed immersed in 60℃ water for 10 min exhibited significantly high germination (80%) than seeds immersed at other temperatures and different duration. The optimum temperature in seed germination was 30℃ and the germination percent was above 80%. Test on seed germination at four media showed that the germination rate was above 80% when seed pre-immersed in 60℃ water for 10 min. Using sand or paper as medium, the germination rate was increased to 90%. Seeds immersed in H2O2 water or GA3 solution could increase the germination of bitter gourd, but not in 98% H2S04.