Distinctness, uniformity and stability (DUS for short) test guidelines are the technical base of plant variety protection (PVP) and the technical manuals for the DUS testing authorities to conduct the testing. The DUS test guidelines for Catasetinae preparation are based on other orchids test guidelines, UPOV test guidelines for Phalaenopsis and other UPOV technical documents, also through collecting and observing on 63 varieties of Catasetinae. Eighty-three characteristics were determined, including 48 asterisked characteristics and 35 supplement characteristics. The grading code and interpretation of characteristics were also elucidated. This study could provide a basis of examination of DUS testing for Catasetinae in the future.