單次運動與負離子暴露對肌肉肝醣儲存量與GLUT4蛋白表現之影響

Effect of Acute Exercise and Negative Air Ion Exposure on Muscle Glycogen Storage and GLUT4 Protein Expression

陳慕聰(Mu-Tsung Chen) ； 李麗珍(Li-Chen Lee) ； 何建德(Chien-Te Ho) ； 黃文成(Wen-Chen Huang) ； 方雩(Yu Fang) ； 陳宗與(Chung-Yu Chen)

《大專體育學刊》 11卷1期 (2009/03) Pp. 117-126

https://doi.org/10.5297/ser.200903_11(1).0009

摘要

研究目的：探討單次運動與負離子暴露對肌肉肝醣儲存量與GLUT4蛋白表現量之影響，同時也觀察負離子暴露與運動兩者之間是否具有交互影響。方法：32隻雌性SD大白鼠共分為4組：控制組(C)、負離子暴露組(N)、運動組(E)和運動加負離子暴露組(NE)。控制組(C)給予正常空氣，負離子組(N)於每立方公分300000-500000個負離子濃度下暴露1小時，運動組(E)為游泳運動3小時，休息45分鐘，再游泳3小時後於正常空氣下休息，運動加負離子暴露組(NE)為游泳運動3小時，休息45分鐘，再游3小時後給予每立方公分300000-500000個負離子濃度下暴露1小時。最後此4組皆給予同樣16小時的時間恢復後收集肌肉組織。肌肉組織將用來分析肌肉肝醣含量及GLUT4蛋白表現量。結果：在腓腸紅肌部分，E組(52.56±1.34μmol/g)、N組(54.74±2.72μmol/g)及NE組(57.63±5.31μmol/g)肌肉肝醣含量明顯高於C組(31.41±1.16μmol/g)。腓腸白肌部分，NE組(64.53±3.07μmol/g)肌肉肝醣含量明顯高於C組(40.63±1.64μmol/g)、E組(44.81±1.93μmol/g)、N組(40.68±0.66μmol/g)，而E組與N組個別與C組比較後並無明顯差異。在腓腸紅白肌GLUT4蛋白表現量部分，4組之間無明顯差異。結論：負離子暴露能明顯提高肌肉肝醣儲存量，但此效應似乎不是透過增加GLUT4蛋白表現所達成。運動與負離子暴露對於肝醣儲存量而言，具有加成效果。

關鍵字

游泳 ； 肝醣超載 ； 葡萄糖轉運體

並列摘要

Purpose: This study was carried out to investigate the effect of acute exercise, negative air ion exposure and their interactive effects on muscle glycogen storage and GLUT4 protein expression. Methods: 32 female SD rats were divided into four groups: control (C), negative air ion exposure (N), exercise (E) and exercise combined with negative air ion (NE). N group was exposed under negative air ion (300000~500000/cm^3) for 1-h. The exercise protocol consisted of two times 3-h swimming intervals, with 45-min rest between the intervals. After swimming, NE group was immediately exposed to the same negative air ion concentration for 1-h. Gastrocnemius muscle tissue was collected from all the groups to determine the levels of glycogen and GLUT4 protein expression after 16-h recovery. Results: In the portion of red gastrocnemius muscle, the glycogen content of E group (52.56±1.34μmol/g), N group (54.74±2.72μmol/g) and NE group (57.63±5.31μmol/g) were significantly higher than C group (31.41±1.16μmol/g). But in white gastrocnemius only NE (64.53±3.07μmol/g) group showed higher glycogen levels than C group (40.63±1.64μmol/g), E group (44.81±1.93μ mol/g) and N group (40.68±0.66μmol/g). However, the level of GLUT4 protein expression was not significantly altered in red and white gastrocnemius muscles of all four groups. Conclusion: Negative air ion exposure could significantly elevate muscle glycogen storage, but this increase effect might not be related to GLUT4 protein expression. Moreover, Exercise combine with negative air ion exposure has an additive effect on muscle glycogen storage.

並列關鍵字

swimming ； glycogen super compensation ； glucose transporter

參考文獻

Bergstorm, J.,Hermansen, L.,Hultman, E.,Saltin B.(1967).Diet, muscle glycogen, and physical performance.Acta Physiologica Scandinavica.71,140-150.

Brozinick, J. T.,Birnbaum, M. J.(1998).Insulin, but not contraction, activates Akt/PKB in isolated rat skeletal muscle.Journal of Biology Chemistry.273(24),14679-14682.

DeFronzo, R. A.,Jacot, E.,Jequier, E.,Maeder, E.,Wahren, J.,Felber, J. P.(1981).The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization.Diabetes.30,1000-1007.

Hallsten, K.,Yki-Jarvinen, H.,Peltoniemi, P.,Oikonen, V.,Takala, T.,Kemppainen, J.,Laine, H.,Bergman, J.,Bolli, G. B.,Knuuti, J.,Nuutila, P.(2003).Insulin- and exercise-stimulated skeletal muscle blood flow and glucose uptake in obese men.Obesity Research.11(2),257-265.

Ivy, J. L.,Brozinick, J. T.,Torgan, C. E.,Kastello, G. M.(1989).Skeletal muscle glucose transport in obese Zucker rats after exercise training.Journal Applied Physioligy.66,2635-2641.