Title

二噁英对小鼠异位子宫内膜影响的分子机制研究

Translated Titles

Study of Molecular Mechanism on Effect of TCDD on Development of Ectopic Endometrium in Mouse

Authors

沈杨(Yang Shen);刘娟(Juan Liu);任慕兰(Mu-Lan Ren)

Key Words

四氯二苯并二噁英 ; 子宫内膜异位症 ; 小鼠 ; AhR ; CYP1A1 ; 2,3,7,8-tetrachiorodibenzo-p-dioxin TCDD ; endometriosis ; mouse ; AhR ; CYP1A1

PublicationName

中山大學學報(醫學科學版)

Volume or Term/Year and Month of Publication

31卷5期(2010 / 09 / 30)

Page #

608 - 613

Content Language

簡體中文

Chinese Abstract

【Objective】 To investigate the effect of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) on development of endometriosis in a mouse model from perspective of molecular mechanism. 【Methods】 The endometriosis mouse model was established with autotransplantation of endometrium. Twenty-one days prior to induction surgery which produces endometriosis, female mice were pretreated with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) at 0, 3, or 10 mg TCDD /kg. Animals were treated again at the time of surgery and at 3, 6, and 9 weeks following surgery. Evaluation of ectopic focuses diameter were made at 3, 6, 9 weeks post surgery. The AhR and CYP1A1 expression on ectopic endometrium were identified by immunohistochemistry and RT-PCR assays. 【Results】 ①With increased time and dose of TCDD exposure, it produced a dose-dependent increase in endometriotic site diameter when all time points were pooled within each dose in mice (r=0.727 and 0.553 respectively, both P<0.05). ②The expression of AhR protein on ectopic focuses in mice were higher in the TCDD exposure group than those in control group, and presented time-dose dependent increase (r=0.687 and 0.442, both P<0.01). ③The higher dose of exposure increased, the higher CYP1A1 protein expressions enhanced (r=0.640, P<0.01). ④As compared with the control group, the expression of AhR mRNA in ectopic focuses of mice were higher in the TCDD exposure group, and show time-dose dependent increase (r=0.565 and 0.635, both P<0.01). ⑤The higher dose of exposure increased, the higher CYP1A1 mRNA expression enhanced (r=0.659, P<0.01). 【Conclusion】 TCDD can promote progression of ectopic focus of endometriosis in the mouse model, and one of molecular mechanism of TCDD for development of EMS is the activated AhR and its reaction gene of CYP1A1.The activated AhR and CYP1A1 can be acted as biochemical indicator to TCDD exposure.

English Abstract

【Objective】 To investigate the effect of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) on development of endometriosis in a mouse model from perspective of molecular mechanism. 【Methods】 The endometriosis mouse model was established with autotransplantation of endometrium. Twenty-one days prior to induction surgery which produces endometriosis, female mice were pretreated with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) at 0, 3, or 10 mg TCDD /kg. Animals were treated again at the time of surgery and at 3, 6, and 9 weeks following surgery. Evaluation of ectopic focuses diameter were made at 3, 6, 9 weeks post surgery. The AhR and CYP1A1 expression on ectopic endometrium were identified by immunohistochemistry and RT-PCR assays. 【Results】 ①With increased time and dose of TCDD exposure, it produced a dose-dependent increase in endometriotic site diameter when all time points were pooled within each dose in mice (r=0.727 and 0.553 respectively, both P<0.05). ②The expression of AhR protein on ectopic focuses in mice were higher in the TCDD exposure group than those in control group, and presented time-dose dependent increase (r=0.687 and 0.442, both P<0.01). ③The higher dose of exposure increased, the higher CYP1A1 protein expressions enhanced (r=0.640, P<0.01). ④As compared with the control group, the expression of AhR mRNA in ectopic focuses of mice were higher in the TCDD exposure group, and show time-dose dependent increase (r=0.565 and 0.635, both P<0.01). ⑤The higher dose of exposure increased, the higher CYP1A1 mRNA expression enhanced (r=0.659, P<0.01). 【Conclusion】 TCDD can promote progression of ectopic focus of endometriosis in the mouse model, and one of molecular mechanism of TCDD for development of EMS is the activated AhR and its reaction gene of CYP1A1.The activated AhR and CYP1A1 can be acted as biochemical indicator to TCDD exposure.

Topic Category 醫藥衛生 > 醫藥衛生綜合