Title

白藜芦醇诱导大鼠原代脂肪细胞凋亡及机制

Translated Titles

Apoptotic Effect and Mechanism of Resveratrol in Rat Primary Adipocytes

Authors

陈思凡(Si-Fan Chen);周妮曼(Ni-Man Zhou);郑琳(Lin Zheng);柯梁汝(Liang-Ru Ke);单智铭(Zhi-Ming Shan);冯翔(Xiang Feng)

Key Words

白藜芦醇 ; 凋亡 ; 沉默信息调节因子1 ; 细胞色素C ; 半胱天冬氨酸酶 ; resveratrol ; apoptosis ; silent information regulator 1 ; cytochrome C ; caspases

PublicationName

中山大學學報(醫學科學版)

Volume or Term/Year and Month of Publication

31卷5期(2010 / 09 / 30)

Page #

619 - 623

Content Language

簡體中文

Chinese Abstract

【Objective】 To explore the effect and mechanism of resveratrol (Res) on apoptosis of rat primary adipocytes. 【Methods】 Rat primary adipocytes were cultured and treated with resveratrol in different dosages. Adipocytes were stained by Hoechst 33258, and cell morphological transformation was examined with fluorescent microscope. DNA Ladder assay was used to further confirm cell apoptosis. The LDH content in medium and cells was measured to calculate the LDH leaking ratio. The number of apoptotic cells were assayed with flow cytometry. The expression of silent information regulator 1(Sirt1), Cytochrome C, Cleaved Caspase 9, Cleaved Caspase 3 were examined using Western blot. 【Results】 Res induced the apoptosis of rat primary adipocytes in a dose dependent manner. Nuclear fragmentation and condensation were observed by Hoechst 33258 staining and DNA Ladder assay. Res also increased the LDH leaking ratio, the number of apoptotic cells and the expression levels of Sirt1 and Cytochrome C, Cleaved Caspase 9, Cleaved Caspase 3. 【Conclusion】 Res could induce the apoptosis of rat primary adipocytes. The underlying mechanisms may be enhancing expression of Sirt1, Cytochrome C, Cleaved Caspase 9 and Cleaved Caspase 3 which are related to cell apoptosis.

English Abstract

【Objective】 To explore the effect and mechanism of resveratrol (Res) on apoptosis of rat primary adipocytes. 【Methods】 Rat primary adipocytes were cultured and treated with resveratrol in different dosages. Adipocytes were stained by Hoechst 33258, and cell morphological transformation was examined with fluorescent microscope. DNA Ladder assay was used to further confirm cell apoptosis. The LDH content in medium and cells was measured to calculate the LDH leaking ratio. The number of apoptotic cells were assayed with flow cytometry. The expression of silent information regulator 1(Sirt1), Cytochrome C, Cleaved Caspase 9, Cleaved Caspase 3 were examined using Western blot. 【Results】 Res induced the apoptosis of rat primary adipocytes in a dose dependent manner. Nuclear fragmentation and condensation were observed by Hoechst 33258 staining and DNA Ladder assay. Res also increased the LDH leaking ratio, the number of apoptotic cells and the expression levels of Sirt1 and Cytochrome C, Cleaved Caspase 9, Cleaved Caspase 3. 【Conclusion】 Res could induce the apoptosis of rat primary adipocytes. The underlying mechanisms may be enhancing expression of Sirt1, Cytochrome C, Cleaved Caspase 9 and Cleaved Caspase 3 which are related to cell apoptosis.

Topic Category 醫藥衛生 > 醫藥衛生綜合