Title

23-plex Y-SNPs复合扩增体系在法医学应用的研究

Translated Titles

Development of 23-plex Y-SNPs Typing Method Based on Single-base Extension and Capillary Electrophoresis Technology and Its Application in Forensic Science

Authors

张爱平(Ai-Ping Zhang);刘超(Chao Liu);李越(Yue Li);刘长晖(Chang-Hui Liu);陈丽伟(Li-Wei Chen);葛璐璐(Lu-Lu Ge);陈晓晖(Xiao-Hui Chen)

Key Words

个体识别 ; 单碱基延伸微测序 ; SNaPshot试剂盒 ; 单核苷酸多态性 ; Y染色体 ; individual identification ; single base extension SBE ; minisequencing ; SNaPshot Kit ; single nuceiotide polymorphysim ; Y chromosome

PublicationName

中山大學學報(醫學科學版)

Volume or Term/Year and Month of Publication

31卷5期(2010 / 09 / 30)

Page #

685 - 691

Content Language

簡體中文

Chinese Abstract

【目的】应用SNaPshot技术建立23-plexY-SNPs的复合扩增体系,研究其在法医学中的应用价值。【方法】从Y染色体单体群命名树上选择23个Y-SNP位点:P164、P203、P148、P145、M89、P151、M216、P128、P157、P149、P131、P199、P123、P191、P201、M111、M9、P132、P200、P197、M119、P136和M134,进行复合PCR扩增,PCR产物纯化后利用荧光标记单碱基延伸技术(SNaPshot试剂盒)结合毛细管电泳技术对单核苷酸多态性进行检测,应用该体系调查了290名广东地区无亲缘关系的男性个体。【结果】所建立的23个Y-SNPs检测体系,各位点均具有遗传多态性,基因多样性范围为0.0137~0.4912。在290名男性中检测到143个单体型,单体型多样性为0.9907。【结论】该23个Y-SNPs检测体系能快速而准确的对样本进行SNP分型,在法医学、人类学和相关学科的研究中具有较高的应用价值。

English Abstract

【Objective】 To develope a robust single nucleotide polymorphism (SNPs) typing assay with co-amplification and to type 23 human Y chromosome SNPs using SNaPshot kit. 【Methods】 Markers were drawn from the phylogenetic tree of Y chromosome: P164, P203, P148, P145, M89, P151, M216, P128, P157, P149, P131, P199, P123, P191, P201, M111, M9, P132, P200, P197, M119, P136, and M134. All 23 Y-SNPs in one reaction were amplified; the pooled PCR products were purified. The minisequencing reactions were performed simultaneously for all 23 Y-SNPs with fluorescein-labeled dideoxynucleotides. A total of 290 male Chinese in Guangdong were genotyped by capillary electrophoresis and multicolour fluorescence detection. 【Results】 A 23-plex Y-SNPs co-amplification system was developed. All of the Y-SNPs are polymorphic in Chinese. The gene diversity of the Y-SNPs ranged from 0.013 7-0.491 2.A total of 143 haplotypes were found, the haplotype diversity was calculated to be 0.990 7. 【Conclusion】 The 23-plex Y-SNPs system developed is efficient, high-throughput and appears to be suitable for forensic science and anthroponomy.

Topic Category 醫藥衛生 > 醫藥衛生綜合