Title

蔗糖诱导型枯草杆菌工程菌高效表达条件研究

Translated Titles

Studies on the High Efficient Expression Condition of B. Subtilis Engineered Strains Induced by Sucrose

Authors

李瑞芳(Rui-Fang Li);李慧琴(Hui-Qin Li)

Key Words

枯草杆菌 ; 蔗糖诱导 ; 高效表达 ; B. subtilis ; sucrose induced ; high efficient expression

PublicationName

河南工業大學學報(自然科學版)

Volume or Term/Year and Month of Publication

26卷6期(2005 / 12 / 20)

Page #

13 - 16

Content Language

簡體中文

Chinese Abstract

为提高枯草杆菌工程菌外源基因的表达水平,探索了蔗糖诱导型枯草杆菌工程菌表达系统DB1342(pSC18-66)的表达条件,筛选到蔗糖诱导型枯草杆菌工程菌最佳表达条件为:在枯草杆菌对数生长前期加蔗糖进行诱导表达,蔗糖的诱导剂量控制在2%~4%,诱导表达时间为20~24h。

English Abstract

To enhance the expression efficiency of heterologous gene in B. subtilis, The conditions of the high efficient expression of B. subtilis engineered strain DB1342(pSC18-66) induced by sucrose was studied. The results showed that sucrose was added at the front middle stage of growth and induced for 20-24 hours with the concentration of 2%~4% were suitable for B. subtilis to express heterologous gene.

Topic Category 基礎與應用科學 > 基礎與應用科學綜合
生物農學 > 生物農學綜合
工程學 > 工程學綜合
工程學 > 化學工業