Effect of Prostaglandin E1 Analogue, Alprostadil, Against the Hepatic Ischemia-Reperfusion Injury in Rats

前列腺素E1類似物(Alprostadil)對於大白鼠肝臟缺血再灌流損傷保護效果之探討

謝政橘(Cheng-Chu Hsieh) ； 邱仁輝(Jen-Hwey Chiu) ； 吳應寧(Ying-Ling Wu)

《臺灣獸醫學雜誌》 36卷1期 (2010/03) Pp. 7-17

https://doi.org/10.7009/TVJ.201003.0007

摘要

實驗的目的在探討前列腺素E1類似物(alprostadil)在大白鼠肝臟缺血再灌流時的影響。使用Sprague-Dawey品系雄性的大白鼠為實驗動物，以外科手術造成肝臟缺血60分鐘後，緊接著再灌流60分鐘。在大白鼠肝臟缺血前30分鐘先給予前列腺素E1(0.05 μg/kg)，持續至肝臟再灌流實驗結束。結果顯示在對照組及給予前列腺素E1組，血清中ALT和AST的含量顯著低於單獨缺血再灌流組。肝臟的超氧化物歧化酶含量，在對照組及給予前列腺素E1組顯著高於單獨缺血再灌流組。肝臟的一氧化氮含量，在對照組及給予前列腺素E1組顯著低於單獨缺血再灌流組。實驗的結果顯示，在肝臟缺血再灌流時期給予前列腺素E1能有效降低肝臟的傷害。

關鍵字

前列腺素E1 ； alprostadil ； 缺血再灌流 ； 超氧化物歧化酶 ； 一氧化氮

並列摘要

This study was aimed to study the effects of Prostaglandin E1 (PGE1) analogue (alprostadil) on hepatic ischemia-reperfusion (I/R) injury in rats. For this purpose, male Sprague-Dawley rats were subjected to 60 minutes of hepatic ischemia followed by 60 minutes of reperfusion period. The rats were injected continuously with PGE1 (0.05 μg/kg) 30 minutes prior to ischemia and alone with reperfusion period. Our results showed that plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were increased in I/R group than in control, and they were decreased in the groups of PGE1 treatment. The level of hepatic superoxide dismutase (SOD) was significantly depressed by I/R and increased by PGE1 treatment. Hepatic nitric oxide (NO) was significantly increased by I/R and decreased by PGE1 treatment. Our findings showed that PGE1 had beneficial improvement to I/R injury, and probably had a pronounced protective effects on the liver.

並列關鍵字

prostaglandin E1 ； alprostadil ； ischemia-reperfusion ； superoxide dismutase ； nitric oxide