Title

Antimicrobial Susceptibility of Riemerella Anatipestifer Isolates from Ducks and Geese and the Mutations of Gyrase Associated with Quinolone Resistance

Translated Titles

鴨、鵝雷氏桿菌抗菌劑感受性與對喹諾酮類藥物抗藥性有關之Gyrase突變

DOI

10.7009/TVJ.201203.0007

Authors

陳燕萍(Yen-Ping Chen);李淑慧(Shu-Hwae Lee);潘銘正(Ming-Jen Pan);陳秋麟(Chiou-Lin Chen);沈瑞鴻(Jui-Hung Shien);張聰洲(Tsung-Chou Chang);蔡向榮(Hsiang-Jung Tsai)

Key Words

抗菌劑感受性 ; gyrase ; 喹諾酮類藥物 ; 抗藥性 ; 雷氏桿菌 ; antimicrobial susceptibility ; gyrase ; quinolones ; resistance ; Riemerella anatipestifer

PublicationName

臺灣獸醫學雜誌

Volume or Term/Year and Month of Publication

38卷1期(2012 / 03 / 01)

Page #

7 - 18

Content Language

英文

Chinese Abstract

This study evaluated the susceptibility of 222 Taiwanese Riemerella anatipestifer isolated from domestic waterfowl between 1999 and 2009 to 22 antimicrobials by agar disk diffusion method. Results showed that most isolates were multi-antimicrobial resistance. R. anatipestifer isolates were most resistant to colistin, nalidixic acid and kanamycin and most susceptible to nitrofurantoin which has been banned for use in domestic animals in 2004, doxycycline and amoxycillin/clavulanic acid. Furthermore, the resistance rates to several antimicrobials and multi-resistance were increased obviously in 2009. In addition, this study investigated the mutations of gyrase associated with quinolone resistance in R. anatipestifer. Most isolates with reduced susceptibility to quinolones possessed amino acid alterations at Ser10→Pro and/or Ser83→Arg or Ile in GyrA and at Thr140→Ile, Ile503→Val and/or Gln155→Val in GyrB. Noteworthily, one isolate with an unusual susceptibility profile which was nalidixic acid-susceptible but enrofloxacin-resistant possessed only one mutation at Gly81→ Asp in GyrA and no change in GyrB. Results of this study can be used to select effective chemotherapies for R. anatipestifer infection in Taiwan. In addition, this study also provides information on a potential association between gyrase mutations and quinolone resistance in R. anatipestifer.

English Abstract

This study evaluated the susceptibility of 222 Taiwanese Riemerella anatipestifer isolated from domestic waterfowl between 1999 and 2009 to 22 antimicrobials by agar disk diffusion method. Results showed that most isolates were multi-antimicrobial resistance. R. anatipestifer isolates were most resistant to colistin, nalidixic acid and kanamycin and most susceptible to nitrofurantoin which has been banned for use in domestic animals in 2004, doxycycline and amoxycillin/clavulanic acid. Furthermore, the resistance rates to several antimicrobials and multi-resistance were increased obviously in 2009. In addition, this study investigated the mutations of gyrase associated with quinolone resistance in R. anatipestifer. Most isolates with reduced susceptibility to quinolones possessed amino acid alterations at Ser10→Pro and/or Ser83→Arg or Ile in GyrA and at Thr140→Ile, Ile503→Val and/or Gln155→Val in GyrB. Noteworthily, one isolate with an unusual susceptibility profile which was nalidixic acid-susceptible but enrofloxacin-resistant possessed only one mutation at Gly81→ Asp in GyrA and no change in GyrB. Results of this study can be used to select effective chemotherapies for R. anatipestifer infection in Taiwan. In addition, this study also provides information on a potential association between gyrase mutations and quinolone resistance in R. anatipestifer.

Topic Category 生物農學 > 動物學
生物農學 > 獸醫
Times Cited
  1. 許庭維(2015)。水禽雷氏桿菌感染菜鴨肝臟初代細胞之模式建立及相關基因表現探討。臺灣大學獸醫學研究所學位論文。2015。1-78。 
  2. 陳燕萍(2012)。鴨、鵝雷氏桿菌抗菌劑感受性與對氯黴素類與奎諾酮類藥物抗藥性機制之研究。臺灣大學獸醫學研究所學位論文。2012。1-133。