The literature indicates that allografts are ideal bone grafts for patients, because they simultaneously exhibit characters of osteogenesis, osteoinduction, and osteoconduction. However, the use of allografts is greatly limited by the lack of a natural supply. In order to provide donor long bone allografts for more patients, allografts should be divided, delipidized, and sterilized; then they can be preserved and utilized. This research aimed to make allografts as particles, delipidized, and sterilized by supercritical carbon dioxide (SCCO2) fluid technology. Research findings showed that allograft particles treated by SCCO2 at 450 bar and 55℃ with the addition of 20% H2O were effectively delipidized and sterilized.