ε-聚離胺酸與磷酸鈣複合材料對於致齲轉醣鏈球菌之抑制作用

The Anti-microbial Ability of ε-polylysine and Calcium Phosphate Complex Material Against Cariogenic Streptococcus Mutans

李盈盈(Yin-Yin Lee) ； 鄧乃嘉(Nai-Chia Teng)

《臺灣兒童牙醫學雜誌》 12卷3期 (2012/12) Pp. 52-58

https://doi.org/10.6319/TJPD.2012.12(3).2

摘要

齲齒在現今的臺灣仍是盛行率極高的慢性疾病之一，許多兒童深受嚴重的蛀牙所苦。轉醣鏈球菌是牙齒表面的群聚前驅菌之一，它能改變齒表環境使後續的菌叢更易積聚造成牙菌斑的形成，增加蛀牙的風險。本研究目的旨在探討一種天然的聚胺基酸: ɛ-聚離胺酸，對致齲菌轉醣鏈球菌的抑制能力。ε-聚離胺酸在日本已被認證為一安全的天然食品抑菌添加物，並被使用於許多不同的抗菌加工產品的製造。本研究將五種不同的飽和鈣鹽溶液: 磷酸二鈣、一水磷酸二氫鈣、磷酸四鈣、碳酸鈣和氯化鈣與5% （w/w）之ε-聚離胺酸混合，將分離出之沉澱物以X光繞射分析作檢測，上清液部份則以瓊脂紙錠擴散法進行抑菌能力測試。實驗結果顯示磷酸二鈣與 0.1 M 磷酸混合加上5% ε-聚離胺酸擁有良好的抑制轉醣鏈球菌能力，並且有最多磷酸鈣的沉澱，未來可望作為一預防齲齒之嶄新配方。

關鍵字

齲齒 ； 抗菌能力 ； ε-聚離胺酸 ； 轉醣鏈球菌

並列摘要

Streptococcus mutans is one of the early colonizers of the tooth surface. The growth and metabolism of the pioneer species changed local environmental conditions enabling more fastidious organisms to further colonization and forming dental plaque. The aim of this study was to investigate the effect of a natural polypeptide product, ε-polylysine, against cariogenic S. mutans. ε-PL is a natural product came from one of the natural human essential amino acids: lysine. It has been approved in Japan as a safe natural food antimicrobial additive, and was widely used on multiple antimicrobial material manufacturing. Our study mixed 5% (w/w) ε-PL with five kinds of saturated calcium salt solutions: DCPD, MCPM, TTCP, Calcium Carbonate and Calcium Chloride. The mixed solutions were centrifuged, the precipitates were examined with XRD analysis and the antimicrobial ability on S. mutans of each treatment solution was tested with agar disc diffusion method. The preliminary results showed that the DCPD+ 0.1 M H3PO4 + 5% ε-PL group have had fair antimicrobial ability and the greatest amount of Calcium Phosphate precipitation.

並列關鍵字

Dental caries ； antimicrobial ability ； ε-polylysine ； Streptococcus mutans