This work uses sprouts cultivation to study sprout growth of electrochemical signals by experimental design for an optimization condition to improve the diameter of sprouts. In this work, five factors (soaking temperature, soaking time, watering frequency, weight pressure and seed density) are studied in this work by FFD and CCD methods.According to the FFD data, soaking temperature and weight pressure are the important key factors to influence the diameter of sprouts. By the CCD data, the watering frequency of six hours, seed density of 150 g, weight pressure of 7.4 Kg and soaking temperature of 46.8℃ to grow the optimal diameter size of sprouts about 2.88 mm.