Comparison of Immunoassay and High Performance Liquid Chromatography Methods for Determining Aflatoxin M1 in Raw Milk

Belkys Xiomara Díaz ； Olga Liliana Rojas ； Isaac Dodino

《Advance Journal of Food Science and Technology》 16卷S期 (2018/11) Pp. 313-316

https://doi.org/10.19026/ajfst.16.5973

摘要

The objective of this study was to compare methods of immunoassay with High Efficiency Liquid Chromatography methods (HPLC) of processing plants of dairy products. Aflatoxins are a group of organic chemical non-protein compounds of low molecular weight, mainly produced by fungi Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. The Aflatoxin M1 (AFM1) is an oxidative metabolite of Aflatoxin B1 (AFB1), produced by animals after the ingestion of this, which appears in milk and urine. The negative impact of aflatoxins in human health is particularly due to their carcinogenicity, by what it is important the detection and quantification of AFM1 in raw milk used as raw material. In the dairy industry it is common to use rapid tests for the determination of the AFM1, but how reliable are these tests? The study discussed 28 raw milk samples taken in times of drought and rain in the South of Cesar, to determine the AFM1 using an immunoassay method with sensitivity of 0.35 μg/kg and for Liquid Chromatography (HPLC) method. The first method data were analyzed with basic descriptive statistics and with the results obtained by chromatography it was estimated the average level of aflatoxin by intervals, AFM1 average estimate was slightly higher in the dry season. It was also noted that results in dry season coincide with the results of the two methods by 42.9% and in the rainy season by 64.3%. The use of immunoassay method has as a disadvantage: the appearance of cross reactions with mycotoxins from the same group.

關鍵字

Aflatoxigenic ； Aspergillus ； carcinogenicity ； metabolite ； mycotoxin ； sensitivity