紙菸菸葉中亞硝胺檢驗方法之建立及背景值調查

本研究以液相層析串聯質譜儀（liquid chromatograph／tandem mass spectrometer，LC／MS／MS）建立檢測紙菸中4種菸草特異性亞硝胺（tobacco specific nitrosamines，TSNAs）之檢驗方法，分別為N-亞硝基降菸鹼（N’-nitrosonornicotine，NNN）、4-甲基亞硝胺-1-3-吡啶基-1-丁酮（4-（methylnitrosamino）-1-（3-pyridyl）-1-butanone，NNK）、N-亞硝基新菸鹼（N’-nitrosoanatabine，NAT）及N-亞硝基新菸草鹼（N’-nitrosoanabasine，NAB）。取均質後之菸葉以乙酸乙酯利用超音波萃取，過濾後濃縮至乾，以甲醇回溶後以LC／MS／MS進行分析，層析分離部分採用Waters Atlantis T3管柱，以含10mM甲酸胺之甲醇及水溶液為移動相進行梯度沖提，質譜分析為利用多重反應偵測模式（multiple reaction monitoring，MRM）進行TSNAs之偵測，回收率介於88.2－113.5%，CV值介於1.4－6.0%均小於10%。應用本研究所建立之方法檢測51件市售紙菸，11件國產紙菸菸葉TSNAs檢出量為0.12－0.69mg／kg，9件大陸紙菸菸葉檢出量為0.03－1.07mg／kg，31件進口紙菸菸葉檢出量為0.08－0.94mg／kg，皆未超過歐盟建議限量標準10mg／kg。

關鍵字

菸草；亞硝胺；液相層析串聯質譜儀

並列摘要

This study investigated 4 tobacco specific nitrosamines (TSNAs) of cigarettes, including N’- nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N’-nitrosoanatabine (NAT) and N’-nitrosoanabasine (NAB) by liquid chromatograph/tandem mass spectrometer (LC/MS/ MS). Homogenized tobacco was extracted with ethyl acetate by ultrasonic. The extract was evaporated to dryness and re-dissolved in methanol prior to analysis on LC/MS/MS. Waters Atlantis T3 column was used for separation with gradient elution between 10 mM ammonium formate and water solution. The mass spectrometry analysis of TSNAs was taken in the multiple reaction monitoring (MRM) modes. The recovery of TSNAs was between 88.2 and 113.5%. Fifty-one commercial cigarettes were investigated by this method. The results showed that the contents of TSNAs in 11 domestic cigarettes was 0.12-0.69 mg/kg, 9 cigarettes imported from China 0.03-1.07 mg/kg and 31 imported cigarettes 0.08-0.94 mg/kg. The contents of TSNAs in tobacco did not exceed the proposed limits 10 mg/kg of European Union.

並列關鍵字

nitrosamines ； LC/MS/MS ； cigarettes

國際替代計量

紙菸菸葉中亞硝胺檢驗方法之建立及背景值調查

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