第一型類胰島素生長因子（IGF-I）對腦轉移肺癌細胞具自主分泌刺激效應

Insulin-like Growth Factor-I Mediates Autocrine Growth Regulation of the Brain Metastatic Variant of Human Lung Cancer Cells in Culture

黃秋琴(Chiu-Chin Hwang) ； 方剛(Kang Fang) ； 施河(Stephen H. Shih)

《師大生物學報》 29卷2期 (1994/12) Pp. 57-64

https://doi.org/10.29812/BBNTNU.199412.0001

摘要

第一型類胰島素生長因子（IGF-I）對細胞之生長發育有重要的調節作用。在許多癌細胞株中，IGF-I也扮演自主分泌刺激（autocrine）的作用。為分析IGF-I在腫瘤形成過程中的變異情形，本文就非細小肺癌細胞株（NSCLC）及其腦移轉細胞株，探討IGF-I及其受體（IGF-I）之基因表現情形。由反轉錄酶帶動熱聚合連鎖反應（RT-PCR）實驗顯示所用的兩種原生非小肺癌細胞株（包括巨大細胞株H460及鱗細胞株H226）僅有極少量IGF-I表現，但肺癌腦轉移細胞株（H226B）則表現出大量的IGF-I mRNA。在受體方面，所有測試細胞株均有相近之IGF-I-R mRNA表現。而以IGF-I-R單株抗體（αIR3）作細胞增殖試驗顯示，H226B之生長會受到抗體增加而有抑制遞增現象，而其原生細胞H226受IGF-I-R抗體影響則不明顯。這些結果顯示出肺癌腦轉移細胞株會分泌IGF-I以調節自身的生長，而在轉移過程後細胞必需依賴以IGF-I主導之自主分泌刺激效應機制。

關鍵字

類胰島素生長因子 ； 自主分泌刺激效應 ； 癌細胞轉移

並列摘要

Insulin-like growth factor I (IGF-I) is important in regulating cell growth and development. IGF-I functions as an autocrine growth stimulus in many cancer cell lines. In order to analyze the function of IGF-I in tumor progression, IGF-I, its receptor gene expression and peptide secretion were examined in non-small-cell lung cancer cell lines (NSCLC) and the brain metastatic variant. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that, compared to two cultured NSCLC cell lines (H226 and H460), the brain metastatic variant H226B showed IGF-I gene expression elevation. All cell lines examined expressed same levels of IGF-I-R gene as demonstrated by RT-PCR. It was also found that αIR3, an IGF-I-R specific antibody, inhibited the growth of H226B. These results suggest that IGF-I is critical in the regulation of cell growth of H226B.The growth of lung tumor brain metastasis progression is regulated by IGF-I-mediated autocrine mechanism.

並列關鍵字

Insulin-like growth factor ； Autocrine ； Metastasis