單分子偵測技術於核酸分子新陳代謝之偵測

Single-molecule Approaches to Study DNA Metabolism

蘇舒(Shu Su) ； 蘇柏宇(Bo-Yu Su) ； 許冠偉(Kuan-Wei Hsu) ； 劉子寧(Zi-Ning Liu) ； 范秀芳(Hsiu-Fang Fan)

《化學》 79卷1期 (2021/03) Pp. 45-58

https://doi.org/10.6623/chem.202103_79(1).012

摘要

細胞內大量的遺傳訊息被保存於核酸分子中，要維持遺傳物質的完整性對於細胞的正常功能是非常重要的。而在細胞內，有各式各樣的調節機制用於維持遺傳物質的完整性，而此一調節機制被概括為核酸分子的新陳代謝。在本文中，我們將介紹數種單分子偵測方法。並介紹如何用單分子栓球技術去研究核酸分子之新陳代謝過程，包含同源重組的反應及調控機制、特定位置重組蛋白的反應及調控機制、染色質的縮合及核小體的結構之調控，得到大量的新訊息證實單分子偵測技術確實對於分子機制的探討為有用的偵測方法。

關鍵字

單分子偵測 ； 同源重組 ； 染色質縮合 ； 核小體結構調控 ； 特定位置基因重組

並列摘要

Single-molecule techniques can observe the individual interaction which has be buried by conventional biochemical techniques. Therefore, single-molecule methods offer valuable complementary information on detailed molecular mechanisms of enzyme functions1-8. There are a lot of breakthroughs in medical, physics, chemistry, biology or biochemistry because of these fast developments of single molecule techniques. For scientists in biochemistry or biophysics, the most popular and well-developed single molecule techniques are bead manipulation methods (such as optical tweezers, magnetic tweezers and tethered particle motion) and fluorescence based techniques (such as fluorescence resonance energy transfer (FRET). In this paper, we will introduce several single-molecule approaches to investigate ＂DNA metabolism＂, including homologous recombination, site-specific recombination, DNA package and nucleosome remodeling process and describe the obtained information.

並列關鍵字

Single-molecule techniques ； Homologous recombination ； Site-specific recombination ； DNA package and nucleosome remodeling process