比咯高分子於微型生醫感測元件之表面改質與細胞生理應用之探討

Studies of Surface Modification for Bio-MEMS Microdevices and Cell by Polypyrrole

李明峰(Ming-Fung Lee)

指導教授 ： 林啟萬

國立臺灣大學/工學院/醫學工程學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00017

摘要

多通道微電極陣列已發展相當多年，其主要目的為刺激與紀錄神經電訊號，而在長時間植入時，其紀錄弁鄔鼎僩|隨時間產生漂移或失效，針對此一問題，對電極進行適當之表面改質以維持細胞與電極間之穩定性，為一合理且有效之方法。導電性高分子因具有易經由電化學聚合方式於金屬表面製備之特性，可避免電極直接與細胞接觸、保護電極、有效降低介面阻抗。此外，電化學聚合方式可配合物理性方式包埋酵素、蛋白質或其他化學單體，輕易地將特定電極或元件表面特性改變。
本研究所發展之電化學表面改質平台，利用比咯高分子電化學沉積方式製作於微感測器元件表面。利用比咯高分子於微機電元件進行表面改質，期以改善靈敏度、促進細胞吸附、保護電極表面以及適當地包埋固定誘導分子、生物分子或酵素作為生物感測器。目前研究結果顯示，可透過循環次數來控制比咯高分子之生成奈米等級膜厚，也可以比咯高分子所具有的包埋效果以及其應用於促進細胞之吸附。
結果分析顯示，在比咯高分子之表面特性分析上，沈積於微電極表面之比咯高分子粗糙度相較其沉積於金膜顯著增加。以及可控制循環聚合次數，每增加一循環膜厚呈現以平均約8.4 nm膜厚遞增；在改質部份，比咯高分子已成它a包埋Streptavidin-FITC、葡萄糖氧化酶以及抗原，而且所包埋之生物分子均有其弁鄔呇s在。此外，與SPR結合進行檢測，發現比咯高分子膜厚每增加8.4 nm會造成SPR角度約0.24度之變化，同時可檢測抗體抗原反應；最後，在細胞生理分析部份，經由比咯高分子包埋吸附蛋白進行改質之樣本表面，相較未改質之表面有兩倍之心臟細胞存活，而且有助微電極陣列於心肌細胞之電訊號量測。

關鍵字

包埋 ； 心肌細胞 ； 比咯高分子 ； 表面改質 ； 多通道微電極陣列

並列摘要

Multichannel neural probes have been fabricated with MEMS technology. The electrodes are involved in the stimulating and recording of impulses from neurons of CNS, PNS. Current problems of chronic recording in CNS is that the device easily loses its ability to record neural activity in days after implantation. Surface modification is critical for maintaining the stable connection between electrodes and cells. Conductive polymer can facilitate the neural signal transduction from the cell to electrode, avoid electrode contact with cells directly, protect electrode, promote cells adhesion and easily fabrication by electrochemical method. More importantly, electrochemical polymerization method could entrap enzyme, protein and functional molecules to change the surface characteristics of electrodes and MEMS devices. In this thesis, an electrochemical deposition method has been developed to deposit conductive polymers together with bioactive molecules onto electrodes surface in nanometer thickness.
We reported the effects of the numbers of CV cycle as a nanofabrication controlling parameter on the deposited film thickness and roughness. The results indicate that the roughness of film deposited on microelectrode is increased dramatically than on macro electrode. In the results of PPy entrapment efficiency, fluorescent molecules entrapment can be demonstrated successfully and entrapped biomolecules remain their activity. And combined with SPR technology, we could detect the thickness of PPy and antigen-antibody interaction. It is noted that when the cardiomyocytes adhere to the foreign material such as electrode, the cell adhesion depends on the surfaces of electrodes and PPy plays a role for the adhesion of cardiomyocytes.

並列關鍵字

PPy, entrap ； cardiomyocyte ； Multichannel neural probes ； Surface modification

參考文獻

[15]陳文章, “導電性高分子＂,化學, 52 (1995), p.197-202.

[13]秦治平, “導電高分子(聚(鄰-胺基苯甲基醚)、聚咇咯) / 蒙脫土奈米複合材料”, 中原大學化學研究所碩士論文, 2001.

[1]Cui Xinyan; Wiler James; Dzaman Marta; Altschuler Richard; Martin David, “In vivo studies of polypyrrole/peptide coated neural probes,” Biomaterials, 24(2003), p777~87.

[2]Cui Xinyan; Hetke Jamille; Wiler James; Anderson David; Martin David, “Electrochemical deposition and characterization of conducting polymer polypyrrole/PSS on multichannel neural probes,” Sensor and Actuators A, 93(2001), p.8-18.

[3]Cui Xinyan; Valerie Lee; Yehoash Raphael; James Wiler; Jamille Hetke; David Anderson; David Martin, “Surface modification of neural recording electrodes with conducting polymer/biomolecule blends,” Journal of Biomedical Materials Research, 2001, p.261-72.