Title

以基因微陣列比較RNA表現發現台灣引起肝膿瘍之克雷伯氏肺炎桿菌與allantoin代謝有關之特有的染色體片段

Translated Titles

Isolation of a Chromosomal Region Associated with Allantoin Metabolism and Liver Infection in Klebsiella pneumoniae by Microarray

DOI

10.6342/NTU.2004.00155

Authors

周慧琦

Key Words

肝感染 ; 克雷伯氏肺炎桿菌 ; 微陣列 ; 肝膿瘍 ; 致病因子 ; microarray ; allantoin ; virulence factor ; liver abscess ; Klebsiella pneumoniae

PublicationName

臺灣大學微生物學研究所學位論文

Volume or Term/Year and Month of Publication

2004年

Academic Degree Category

博士

Advisor

王錦堂

Content Language

繁體中文

Chinese Abstract

克雷伯氏肺炎桿菌(Klebsiella pneumoniae)所引發的原發性肝膿瘍 (primary liver abscess),目前已成為台灣成人常見的社區感染症之一,然而其致病機轉尚未完全明瞭。因此,我們利用基因微陣列 (microarray)來尋找可能存在於引起肝感染克雷伯氏肺炎桿菌 (liver infection K. pneumoniae) 特有的致病因子(virulence factor)。比較引起肝感染與不引起肝感染 (non-liver infection) 克雷伯氏肺炎桿菌RNA表現的差異,發現於肝感染克雷伯氏肺炎桿菌表現值大於非肝感染克雷伯氏肺炎桿菌表現值兩倍以上者有14個殖株 (clones)。其中3個殖株(clone-1, 2, 6)為相同,並且5’ 端有1374 bp的DNA序列無法在克雷伯氏肺炎桿菌MGH 78578找到相對應的位置。菌株MGH 78578為非肝感染菌株,其基因體序列解碼目前正由美國華盛頓大學基因體定序中心 (http//genome.wustl.edu/gsc) 進行中。 DNA定序的結果顯示MGH 78578染色體上一段149 bp的片段在肝感染克雷伯氏肺炎桿菌NTUH-K2044被一段總長21745 bp的片段所取代。此21745 bp片段總共包括19個開放讀架 (open reading frame),其中14個開放讀架證明和allantoin代謝相關。另一方面,這個片段於肝感染及非肝感染克雷伯氏肺炎桿菌的分佈情形有極顯著的差異 (19/32比2/94, p=0.0001, x2 test)。同時,在動物實驗也發現,同樣以胃內感染方式注射104 CFU時,NTUH-K2044在老鼠能造成嚴重的肝膿瘍及致死,而在NTUH-K2044染色體上將具有正向調控allantoin operons弁鄋滌穧]allS作基因剔除 (knock out) 所得的缺陷株 K2044(

English Abstract

Klebsiella pneumoniae liver abscess with metastatic complications is an emerging infectious disease in Taiwan. To identify genes associated with liver infection, we used a DNA microarray to compare the transcriptional profiles between three strains causing liver abscess and three strains without liver infection. There were 14 clones that revealed higher RNA expression levels in the three liver infection strains and three of these 14 clones contained a region that was absent in MGH 78578. The strain of K. pneumoniae MGH 78578 was isolated from a patient without liver abscess and was chosen for full genome sequencing by the Genomic Sequencing Center at Washington University (St. Louis, MO) (http//genome.wustl.edu/gsc). Sequencing of the clones revealed the replacement of 149 bp of MGH 78578 with a 21,745 bp fragment in a liver infection strain, NTUH-K2044. This 21,745-bp fragment contained 19 open reading frames, 14 of which were proven to be associated with allantoin metabolism. The K2044(

Topic Category 醫藥衛生 > 基礎醫學
醫學院 > 微生物學研究所
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Times Cited
  1. 鐘純珊(2009)。克雷白氏肺炎桿菌NTUH-K2044 中纖毛黏附蛋白KpfD的功能性分析。交通大學分子醫學與生物工程研究所學位論文。2009。1-54。