恆春福木葉部之化學成分研究

Studies on the chemical constituents of the leaves of Garcinia multiflora ( Champ. )

林明園(Ming-Yuan Lin)

指導教授 ： 郭悅雄

國立臺灣大學/理學院/化學研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.00184

摘要

恆春福木 ( Garcinia multiflora ) 為金絲桃科藤黃屬的木本植物，又名山桔子，分布於兩廣、福建、以及台灣南部恆春半島低海拔山麓。其樹皮澀、微苦；而果甘酸，性涼，有小毒，能消炎止痛、治燒燙傷、口腔炎及爛瘡等。
本研究為分析恆春福木葉部之化學成分，以甲醇浸泡得到之萃取物利用矽膠管柱層析、矽膠薄層層析、高效能液相層析 ( HPLC ) 及再結晶等方法，做分離純化。純化後的化合物，再利用紅外線光譜、氫核磁共振光譜、碳核磁共振光譜、二維核磁共振圖譜及質譜來推測其結構；若推測的結構經化學資料庫查詢為已知化合物，則與文獻值比對確認，若為尚未被發表過的新化合物，或屬天然物之首次分離，則以二維核磁共振圖譜 ( 2D-NMR ) 及高解析質譜輔助，來進行結構的鑑定。

本實驗由恆春福木葉部所得到研究成果共計45個化合物，分類為：
一. Apocartenoids類 3個
二. 芳香族類 ( Aromatic compounds ) 13個
三. 葉綠素類 ( Chlorophyll ) 2個
四. 木酚素類 ( Lignoids ) 1個
五. 其他類 ( Others ) 7個
六. 類固醇類 ( Steroids ) 8個
七. 三萜類 ( Triterpenoids ) 10個
八. 含口山峒類 ( Xanthones ) 1個
其中8個為新化合物，1個為天然界之首次分離。

關鍵字

福木 ； 葉部 ； 核磁共振圖譜 ； 層析 ； 結構鑑定

並列摘要

The plant of Garcinia multiflora ( Champ. ) distributed over mainland china and Taiwan South is a woody plants. The fruits could been anti-inflammation, anti-scald and anti-stomatitis in medical treatment.
The methanol extract from the leaves of Garcinia multiflora ( Champ. ) was concentrated to give a residue which was suspended in water and then partitioned with ethyl acetate. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography, and high performance liquid chromatography ( HPLC ) to give forty-five components. Among these components, eight are new, and one was isolated for the first time from natural sources. The total isolated components included three apocartenoids, thirteen aromatics, two chlorophyll, one lignoids, seven others, eight steroids, ten triterpenoids, and one xanthone.
By means of IR spectra, 1H-NMR spectra, 13C-NMR spectra and mass spectra, the known compounds were identified with those their physical data which reported in literatures or from authentic samples. The new compounds were elucidated by the using of spectral evidence including 1D and 2D techniques.

The total isolated components have forty-five from the leaves of Garcinia multiflora in my study, and are classified：
一. Apocartenoids ( Three )
二. Aromatics ( Thirteen )
三. Chlorophyll ( Two )
四. Lignoid ( One)
五. Others ( Seven )
六. Steroids ( Eight )
七. Triterpenoids ( Ten )
八. Xanthone ( One )
Among these components, eight are new, and one was isolated for the first time from natural sources.

並列關鍵字

Garcinia multiflora ； HPLC ； NMR ； chromatography

參考文獻

46. Chang, M. H.; Wang, G. J.; Kuo, Y. H.; Lee, C. K. J. Chin. Chem. Soc. 2000, 47, 1131-1136.

53. Chang, M. H.; Wang, G. J.; Kuo Y. H.; Lee, C. K. J. Chin. Chem. Soc. 2000, 47, 1131-1136.

3. Chen, J. J.; Chen, I. S.; Duh, C. Y. Planta Med. 2004, 70, 1195-1200.

33. Chang, Y. C.; Chang, F. R.; Wu, Y. C. J. Chin. Chem. Soc. 2000, 47, 373-380.

36. Pan, W. B.; Wei, L. L.; Wu, C. C.; Chang, F. R.; Wu, Y. C. J. Chin. Chem. Soc. 2005, 52, 581-588.