合成碲化鎘量子點應用於標定孕烯醇酮結合蛋白質之細胞顯影

Synthesis of CdTe Quantum Dots for Pregnenolone Binding Proteins Targeting and in Vivo Imaging

劉書瑋(Shu-Wei Liu)

指導教授 ： 陳昭岑

國立臺灣大學/理學院/化學研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.01057

摘要

半導體材料所製成之奈米粒子量子點 (quantum dots)，因其放射波狹窄對稱與可調控性、吸收係數大、光穩定性佳、螢光量子效率高等光物理特性，相較於傳統有機染料有更多生醫應用之優點。藉由表面生物分子之修飾，可應用於生物標定與細胞顯影之上。胚胎細胞之遷移過程在器官發生與生物體形態之形成上是相當重要的課題。本實驗室先前研究指出，固醇類激素-孕烯醇酮 (pregnenolone) 具有扮演維持微小管穩定與促使細胞遷移之角色。為顯現孕烯醇酮與孕烯醇酮結合蛋白質 (pregnenolone binding proteins, PBPs) 之作用，運用表面修飾孕烯醇酮之量子點，來顯現其在細胞內的相對位置與情況。因此，合成了一系列水溶性量子點，表面包覆具有不同官能基之親水性配體，其一端之二氫硫辛酸接合於量子點表面，另一端為氨基或羥基。具有氨基之配體與孕烯醇酮衍生物形成醯胺鍵，使孕烯醇酮修飾於量子點上，希望能藉此來標定與追蹤細胞內孕烯醇酮結合蛋白質的位置。

關鍵字

量子點 ； 生物分子修飾 ； 孕烯醇酮 ； 生物標定 ； 細胞顯影

並列摘要

The semiconductor nanocrystal quantum dots (QDs) are versatile fluorophores with unique photophysical properties, including narrow and size-dependent luminescence, broad absorption spectra, and high quantum yields. Bioconjugation of surface functionalized QDs is applied to biological imaging and cellular tracking.1 Embryonic cell movement is essential for morphogenesis and the establishment of body shapes. In previous research, we showed that the steroid hormone, pregnenolone, preserves microtubule abundance and promotes cell movement during epiboly.2 To visualize the interaction between pregnenolone and pregnenolone binding proteins (PBPs) on microtubules, the pregnenolone-conjugated QDs are fabricated as a tool to evidence the pregnenolone-related biological events. Hence, we synthesized a series of functionalized water-soluble QDs with heterobifunctional ligands incorporating dihydrolipoic acid (DHLA), a hydrophilic spacer, and a functional (amino) or nonfunctional (hydroxy) terminus. The length of hydrophilic spacer is studied by comparing triethylene glycol (TEG) spacer and poly(ethylene glycol) (PEG) spacer. The covalent conjugation will be finally achieved by amide coupling between functional ligands and pregnenolone derivatives. It is hoped tracking the PBPs in embryonic cells of zebrafish can be achieved with these pregnenolone-conjugate QDs.

並列關鍵字

quantum dots ； bioconjugates ； pregnenolone ； biological labelling ； cellular imaging

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