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  • 學位論文

錫蘭橄欖葉抗氧化成分之研究

Studies on the Antioxidative Constituents of Elaeocarpus serratus Leaves

指導教授 : 張惠婷

摘要


摘要 本研究的目的是評估錫蘭橄欖(Elaeocarpus serratus L.)葉乙醇萃取物的抗氧化活性,進一步分離與鑑定具抗氧化功能的化合物,期望能開發錫蘭橄欖葉萃取物於保健用途上。試驗結果顯示錫蘭橄欖葉乙醇萃取物的總酚類含量為114.16 mg of GAE/g,並具有良好的抗氧化活性,在濃度為19.5 µg/mL時,即可達到50%的DPPH自由基抑制效果;對於超氧自由基的抑制,乙醇萃取物的EC50值為13.67 µg/mL;而總抗氧化能力的測定中,乙醇萃取物的TEAC值為0.46 mM。另外,各可溶部中總酚類的含量以乙酸乙酯可溶部含量達220.6 mg of GAE/g為最高,而且在DPPH自由基抑制能力、還原力、超氧自由基抑制能力和總抗氧化能力等抗氧化能力的測定中,乙酸乙酯可溶部皆顯示最佳的效果。 將最具抗氧化活性的乙酸乙酯可溶部進行管柱層析得到13個次分離部,在DPPH自由基抑制能力的測定中以E8和E9具有最佳效果。以高效能液相層析儀分離與純化具有抗氧化活性的次分離部,共獲得Gallic acid、Myricetin、Mearnsetin、Mearnsitrin和Myricitrin。經過定量後,得知此5個化合物在乙酸乙酯可溶部中,以Gallic acid含量最高,其次為Myricetin和Meanrnsetin,Mearnsitrin和Myricitrin的含量較低。 評估化合物的抗氧化活性,DPPH自由基抑制能力依序為Gallic acid≒Myricetin>Myricitrin>Mearnsetin>Mearnsitrin。還原力評估結果和DPPH自由基抑制能力相同。超氧自由基抑制能力則是以Mearnsetin有最佳的效果,其次為Gallic acid和Myricitrin。總抗氧化能力中,化合物的TEAC值依序為Mearnsetin>Gallic acid> Myricetin>Myricitrin>Mearnsitrin。綜合上述試驗顯示Gallic acid、Myricetin和Mearnsetin此3個化合物在抗氧化活性上有極佳的表現,推測錫蘭橄欖葉乙醇萃取物的抗氧化活性與此3個化合物有關。 本研究證實錫蘭橄欖葉萃取物具有良好的抗氧化活性,並含有強抗氧化物質,顯示錫蘭橄欖除了觀賞與果實利用外,如能朝天然抗氧化物或保健食品等高經濟價值的方向開發,將具有良好的發展潛力。

並列摘要


Abstract The purposes of this study are to evaluate the antioxidative avtivity of Ceylon olive (Elaeocarpus serratus L.) leaves extract and to isolate the antioxidant constituents from leaves extract. It would be helpful to expand the utilization of Ceylon olive leaves for the natural health products. Result demonstrated phenolic compounds were founded rich in E. serratus leaves ethanolic extract with 114.16 mg of GAE/g, and the EC50 value of DPPH radical scavenging and superoxide anion scavenging was 19.5 µg/mL and 13.67 µg/mL. In the total antioxidant assay, E. serratus leaves extract also showed excellent performance with the TEAC value of 0.46 mM. EtOAc soluble fraction show the highest amount of phenolic compounds (220.6 mg of GAE/g) among the fractions, and its also showed the best performance in DPPH radical scavenging assay, reducing power assay, superoxide anion scavenging assay, and total antioxidant assay. EtOAc soluble fraction was then separated into 13 subfractions. Subfraction E8 and subfraction E9 exhibited the best antioxidant activities in DPPH radical scavenging assay. Five compounds, including gallic aicd, myricetin, mearnsetin, mearnsitrin and myricetrin were isolated from active subfractions by HPLC. After quantification, the content of gallic acid was the highest content in EtOAc soluble fraction among the isolated compounds, followed by myricetin and meanrnsetin, mearnsitrin and myricitrin showed the lower content than the others. The antioxidant activities of isolated compounds were also evaluated. The DPPH radical scavenging activities of isolated compounds were in a decreasing order of gallic acid ≒ myricetin>myricitrin>mearnsetin>mearnsitrin, and this result was consistent with that of reducing power assay. Mearnsetin showed the best inhibitory effect in superoxide anion scavenging activity, followed by gallic acid and myricitrin. In the total antioxidant assay, TEAC values of compounds were in a decreasing order of mearnsetin>gallic acid>myricetin>myricitrin>mearnsitrin. Results obtained above indicated that gallic acid, myricetin and mearnsetin possessed great antioxidant activities, and we assumed that the antioxidant activity of E. serratus leaves ethanolic extract might contribute from these compounds. Results revealed that E. serratus leaves extract showed excellent antioxidant activity, and possessed compound with great antioxidant characteristic. Therefore, besides the utilization of ornamental trees and fruits of E. serratus, leaves of E. serratus has the potential to develop the application for the natural health-care food.

參考文獻


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