基質硬度、靜水壓、滲透壓對細胞移動和型態之影響

Effects of Substrate Stiffness, Hydrostatic Pressure, and Osmolarity on Cell Migration and Morphology

葉衍陞(Yen-Sheng Yeh)

指導教授 ： 郭柏齡

國立臺灣大學/電機資訊學院/生醫電子與資訊學研究所/碩士(2013年)

https://doi.org/10.6342/NTU.2013.02294

摘要

細胞在生物體內的生長發育、分化和移動除了受到生物化學刺激之外，物理性刺激如細胞外基質硬度以及滲透壓亦對細胞行為有很大影響。在我們的實驗當中，我們將H9C2肌肉母細胞培養在不同硬度細胞外基質上並且分成對照組和水壓組來探討此肌肉母細胞同時受細胞外基質軟硬度和靜水壓的刺激對細胞分化和細胞型態的影響。另一方面，我們也探討T細胞在二維和三維細胞培養環境微流道中在均勻和梯度滲透壓的情況下，細胞的遷移是否會受到影響。
我們使用聚丙烯醯胺(Polyacrylamide, PA)水膠來當作細胞外基質材料，因為PA水膠的硬度能夠透過調配不同比例的acrylamide 和bis-acrylamide 來製作出不同軟硬度的水膠。H9C2細胞培養於蛋白質fibronectin 附著的水膠表面再分成控制組和10公分靜水壓組並培養48小時後，觀測細胞分化採轉譯因子MyoD表現量和細胞型態。實驗結果顯示，MyoD的表現量在水壓組皆高於控制組，而細胞面積在經過水壓刺激後亦會較控制組來的大，但細胞長寬比則較無明顯差異。因此，靜水壓可能為促進H9C2細胞分化和增加細胞面積的其中一種刺激因素。
在細胞遷移實驗方面，我們將T細胞培養於三種不同滲透壓培養液內(260、337、以及415 mOsm)並計算細胞移動速度。初步實驗結果顯示T細胞在低滲透壓的細胞培養液中，其移動速度會較慢。此外，我們亦製作出兩種微流道來產生滲透壓梯度以觀察T細胞是否會受到滲透壓梯度刺激而影響細胞遷移行為。此兩種裝置分別為二維細胞培養環境的agarose-based微流道和三維細胞培養環境的collagen-filled微流道。滲透壓梯度於三維細胞培養環境微流道能產生約0.031mM/μm的滲透壓梯度，而T細胞在這兩種流道裝置內分別給予均勻和梯度滲透壓環境來觀察細胞的遷移行為。在有滲透壓濃度梯度的環境中並無觀察到細胞會有特定的遷移趨向性。

關鍵字

靜水壓 ； 細胞外基質 ； 軟硬度 ； 細胞遷移 ； 微流道 ； 滲透壓

並列摘要

The cell growth, proliferation, differentiation, and migration are affected by biochemical and biophysical cues, such as the stiffness of extracellular matrix (ECM) and osmolarity. In this work, we studied the effects of hydrostatic pressure and substrate stiffness on the differentiation and morphology of H9C2 myoblast. We also investigated the effects of homogeneous and gradient osmolarity on T cell migration using 2D and 3D microfluidic devices. Polyacrylamide (PA) hydrogel was used as cell culturing substrates and the stiffness of the PA gel was modulated by mixing different ratios of acrylamide to bis-acrylamide. The cell size, aspect ratio, and expression of transcription factor MyoD were measured after culturing the H9C2 cell on fibronectin coated PA gel with and without the presence of 10 cm hydrostatic pressure for 48 hours. The results reveal that the 10-cm-hydrostatic- pressure exposure increased the MyoD expression and cell size, but resulted in insignificant shape change when compared with cells cultured without presence of the pressure. These findings indicate that the hydrostatic pressure promotes the MyoD expression and increases the cell area. As for the cell migration studies, we cultured QL-9 T cells in medium of osmolarity 260, 337, and 415 mOsm. Live cell images were acquired and analyzing the images revealed that the hypotonic solution significantly decreased the migration speed of the T cells. Two microfluidic-based devices were herein designed for studying the cell migration in response to osmotic gradient: one was a three-chambers design made of agarose for studying the cell migration in a 2D plane; another was a two-chambers device with a collagen-filled tunnel in between for investigating cell movement in a 3D space. The osmotic gradient in the collagen-filled tunnel was about 0.031mOsm/μm. Preliminary results showed that such an osmotic gradient did not evoke gradient-directed movement in T cells.

並列關鍵字

hydrostatic pressure ； extracellular matrix ； stiffness ； cell migration ； microfluidics ； osmolarity

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