我們成功的合成出綠色螢光蛋白質(Green Fluorescent Protein,GFP)中心發色團(chromophore) 4-(4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (p-HBDI)的結構異構物4-(2-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (o-HBDI)。o-HBDI擁有七員環氫鍵系統,以致激發態分子內質子轉移(Excited-State Intramolecular Proton Transfer,ESIPT),放射605 nm的螢光。
A structural isomer of the core chromophore (p-HBDI) in green fluorescence protein, o-HBDI, is synthesized. o-HBDI possesses a seven-membered ring hydrogen bond, from which the excited-state intramolecular proton transfer takes place, resulting in a remarkable tautomer emission of ~ 605 nm..