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  • 學位論文

飼料中添加螺旋藻粉及重組類胰島素成長因I 對菊池氏細鯽成長之影響

Effects of feed supplementing with Spirulina meal (Spirulina platensis) and recombinant insulin-like growth factorⅠ on growth performance of Kikuchi minnow (Aphyocypris kikuchii)

指導教授 : 廖文亮

摘要


菊池氏細鯽(Aphyocypris kikuchii),為台灣特有種。常見於台灣東部的宜蘭、花蓮及台東的河川、湖沼等流速較緩的水域中。但是近年來由於人們對於其需求大增而過度捕撈,加上棲地破壞,使其於天然水域中大量減少。目前花蓮縣水產培育所已透過人工苗的生產放流進行復育,對此魚種飼料的開發將有助於復育工作。螺旋藻(Spirulina platensis)有很高的蛋白質含量(60-70%),胺基酸組成與其他成本較高之飼料原料相似,另亦含豐富的維他命、礦物質、必需脂肪酸(γ-亞麻油酸,γ-linolenic acid—GLA)以及抗氧化色素例如類胡蘿蔔素,因此經常被用作飼料營養添加或甚至取代某些原料。 實驗一探討飼料中添加螺旋藻粉(Spirulina platensis)取代玉米筋質粉對菊池氏細鯽魚成長之影響。共有5組,分別為-玉米筋質粉20%、螺旋藻粉0%,Spi.0;玉米筋質粉15%、螺旋藻粉5%,Spi.5;玉米筋質粉10%、螺旋藻粉10%,Spi.10;玉米筋質粉5%、螺旋藻粉15%,Spi.15;玉米筋質粉0%、螺旋藻粉20%,Spi.20。實驗的菊池氏細鯽平均初始體重0.60 ± 0.09g,每組15隻3個重複為期9週。結果顯示,Spi.15組有最高增重率、飼料效率、蛋白質效率及每日氮增量,皆較對照組佳,各組皆無死亡。 由於文獻中皆指出此魚種有生長較緩慢的情形,因此於實驗二各添加0.5%重組類胰島素成長因子I,飼料其餘成分比例皆與實驗一各組相同,探討對菊池氏細鯽成長的影響。實驗魚隻平均初始體重0.78±0.04g,每組10隻3個重複為期9週。結果顯示,Spi.15組同樣於各項數據有最佳表現。 實驗結果顯示,無論飼料中是否添加成長因子,以螺旋藻粉含量15%的Spi.15組之各項數據皆為最佳,而添加重組類胰島素成長因子I的實驗二各組皆較實驗一的相對應組別有明顯較佳增重率。因此推測以螺旋藻粉取代15%的玉米筋質粉對菊池氏細鯽有最佳成長效果,且若飼料中添加微量成長因子對菊池氏細鯽的成長亦有明顯助益。

並列摘要


The first part of this study evaluate the effects of diets containing 20% of corn gluten meal, and substituting 0, 25, 50, 75 and 100% Spirulina platensis for that, i.e. Spirulina platensis accounts for 0, 5, 10, 15, 20% of the feed formula respectively, on growth performance of kikuchii minnow (Aphyocypris kikuchii). A completely randomized experimental design is developed with the abovementioned five treatments and three replicates. 225 kikuchii minnow with average initial weight of 0.60 ± 0.09g are assigned to 5 experimental tanks. The experiment lasts for 9 weeks. Kikuchii minnows fed with 15% S. platensis show a significantly higher weight gain、feed efficiency、protein efficiency ratio and daily N increase than those which are fed with 0% S. platensis (p<0.05). According to previous studies, kikuchii minnow often has a relatively lower growth rate. That further leads to the second part of this study, which not only contains the same 5 groups of feed formula as the first one, but supplements each one with 0.5% recombinant insulin-like growth factor Ⅰ(IGF-Ⅰ). The average initial weight of 150 kikuchii minnow is 0.78±0.04g. The group fed with 15% S. platensis also shows significantly higher performances in all parameters than those which are fed with 0% S. platensis (p<0.05). Both parts of the study show no casualty. The present results demonstrate that S. platensis could be introduced as a substitute in kikuchii minnow diets without compromising their growth and might even be a booster. The improved growth and feed utilization are probably due to enhancement of protein assimilation. As the results indicate, the proper dietary Spirulina meal level is 15%. In addition, supplementing with 0.5% IGF-Ⅰwill cast a positive effect on their growth performance.

參考文獻


蔡欣原 (2010) 飼料中添加重組類胰島素成長因子及植物性原料取代魚粉對銀鱸與寶石鱸成長之影響。國立台灣大學漁業科學研究所碩士論文。pp. 43.
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陳威廷 (2012) 飼料中添加不同來源之脂質在不同溫度對菊池氏細鯽成長之影響。國立台灣大學漁業科學研究所碩士論文。pp. 43.
陳柔伊 (2007) 飼料中添加重組類胰島素成長因子對日本鰻與筍殼魚成長
蘇柏安 (2006) 飼料中添加重組類胰島素成長因子與植物性原料取代魚粉對赤鰭笛鯛與海鱺成長之影響。國立台灣大學漁業科學研究所碩士論文。pp. 87.

被引用紀錄


吳佳穎(2015)。飼料中添加狹葉羽扇豆粉取代玉米筋質粉對菊池氏細鯽成長之影響〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2015.02884

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