Title

潺槁樹葉部抗發炎成分之研究

Translated Titles

Study on the Anti-inflammatory Constituents from Litsea glutinosa Leaves

DOI

10.6845/NCHU.2007.00019

Authors

羅昱超

Key Words

潺槁樹葉 ; 抽出成分 ; 抗發炎 ; L. glutinosa ; Leaves ; Extractives ; Anti-inflammatory activity ; Methyl-p-hydroxycinnamate

PublicationName

中興大學森林學系所學位論文

Volume or Term/Year and Month of Publication

2007年

Academic Degree Category

碩士

Advisor

呂金誠

Content Language

繁體中文

Chinese Abstract

潺槁樹為金門地區常見的原生樹種,由於其對當地之各項立地適應力極強,天然更新容易,因此成為金門主要之保安林樹種,並於1986年代後引進臺灣栽植,成效良好。在傳統用藥及文獻報告中皆顯示潺槁樹成分具有抗發炎的活性,然相關的科學證據仍顯不足。為促增潺槁樹之經濟價值,本研究乃針對其葉部之抽出物進行抗發炎活性評估,結果顯示,潺槁樹抽出物具有極佳的抗發炎能力,並以乙酸乙酯可溶部對一氧化氮自由基之清除效果最好。本研究續利用管柱層析將乙酸乙酯可溶部分離成15個次分離部,其中以EtOAc-6、EtOAc-7及EtOAc-13等次分離部具有良好的抑制NO自由基生成活性。又利用高效能液相層析儀自EtOAc-6次分離部中純化出其主要成分-對羥基肉桂酸甲酯(MHC),由結構-活性相關性研究之結果得知,MHC為同類衍生物中對NO自由基清除能力最佳的化合物,其IC50為12.51 μg/mL。抑制LPS誘導巨噬細胞產生PGE2之試驗中,結果顯示MHC在濃度為25 μg/mL時具有顯著抑制PGE2的生成。在iNOS及COX-2蛋白表現的試驗中,結果顯示MHC可經由IKK/IκB/NF-κB路徑調控iNOS蛋白的表現,並進而抑制NO自由基的生成。另外,利用巴豆油誘導ICR小鼠耳朵發炎之動物試驗中顯示,MHC抑制鼠耳發炎水腫的能力已相當於非類固醇類抗發炎藥(NSAID) indomethacin之效果。綜合上述結果,潺槁樹EtOAc-6所分離之主要成分MHC極具有開發成為抗發炎藥物之潛力。

English Abstract

Litsea glutinosa (Lour.) C. B. Rob. is a indigenous tree species grown in Kinmen. Due to its excellent adaptability to various habitat and well naturally regeneration property, L. glutinosa is one of the important planted tree species for protection-forest in Kinmen. According to the ethnopharmocological recording, L. glutinosa posses the strong anti-inflammatory activity. However, it lacks scientific study on the bioactivity of L. glutinosa. To enhance the economical value of L. glutinosa, it is worthy to investigate the bioactive compounds of L. glutinosa. In this study, we evaluated the anti-inflammatory activity of L. glutinosa leaves extracts. The result revealed that the extracts possessed a significant anti-inflammatory activity, and the ethyl acetate fraction (EtOAc) presented the strongest inhibitory activity on production of nitric oxide (NO) radical production. The EtOAc fraction was then separated by column chromatography (CC) to derivated into 15 subfractions. Subfractions EtOAc-6, EtOAc-7 and EtOAc-13 showed the potent inhibitory activity on NO radical production. A major compound, methyl-p-hydroxycinnamate (MHC), was isolated from active subfractions EtOAc-6 by high performance liquid chromatography (HPLC). MHC presented the best NO inhibition activity compared with analogic structure, and the IC50 value was 12.51 μg/mL. Results from inhibition of PGE2 production in LPS-stimulated macrophages demonstrated that MHC caused significant inhibition at dosage of 25 μg/mL. Furthermore, results from the protein express assay demonstrated that MHC suppressed iNOS protein expression via IKK/IκB/NF-κB pathway in a dose-dependent manner, and then suppress the formation of NO radical. MHC also exhibited a significant anti-inflammatory activity which compared with indomethacin in croton oil-induced ear edema assay. Results obtained in this study indicated that MHC which isolated EtOAc-6 of L. glutinosa extract has a good potential to develop as an anti-inflammatory agent in the future.

Topic Category 農業暨自然資源學院 > 森林學系所
生物農學 > 森林
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