Title

明道一號薑黃對 3T3-L1 前脂肪細胞及高脂飲食誘導肥胖 BALB/c 小鼠不易形成體脂肪之探討

Translated Titles

Preventive effect of fat accumulation by Curcuma longa L. (MingDao No. 1 turmeric) rhizomes in 3T3-L1 preadipocytes and high fat diet-induced obese BALB/c mice

Authors

邱珮瑄

Key Words

明道一號薑黃 ; 薑黃素 ; 肥胖 ; 3T3-L1 ; 前脂肪細胞分化 ; 高脂飲食 ; BALB/c 小鼠 ; Curcuma longa L. (MingDao No. 1 turmeric) ; curcumin ; obesity ; 3T3-L1 ; preadipocyte differentiation ; high-fat diet ; BALB/c mice

PublicationName

中興大學食品暨應用生物科技學系所學位論文

Volume or Term/Year and Month of Publication

2014年

Academic Degree Category

碩士

Advisor

蔣慎思

Content Language

繁體中文

Chinese Abstract

肥胖是指能量攝取與消耗無法達到平衡,造成脂肪過度的堆積於脂肪組織,使得罹患疾病的風險增加,進而影響健康的一種狀態。與肥胖相關的疾病包括糖尿病、非酒精性脂肪肝、血脂異常、高血壓及心血管疾病等。在肥胖之發展過程中,前脂肪細胞的分化是決定成熟脂肪細胞數目之主要因素。本研究目的為探討明道一號薑黃精油(MingDao No. 1 turmeric essential oil, MDTE)抑制3T3-L1前脂肪細胞分化及抑制油滴堆積之效果。在體外細胞試驗中,以薑黃素(curcumin, tCur)、芳香薑黃酮(ar-turmerone, aTur)及MDTE處理3T3-L1 前脂肪細胞,於分化後第九天,利用油紅染色方法分析3T3-L1成熟脂肪細胞內油滴堆積之情形,並進行脂解作用與脂質生合成活性分析來評估成熟脂肪細胞甘油釋放量與肝素釋放脂蛋白脂解酶 (heparin-releasable LPL, HR-LPL)活性,並配合即時聚合酶鏈鎖反應(real time-polymerase chain reaction,RT-PCR)結果分析相關基因之表現量。結果顯示,20 μM tCur、1 μM aTur及20 ~ 50 μg/mL MDTE 處理後可顯著抑制細胞中油滴堆積(p<0.05),且 MDTE 可藉由向上調控脂解活性來增加甘油釋放量。20 μM tCur、1 μM aTur 及 50 μg/mL MDTE 處理後可顯著減少脂肪細胞分化期間之相關基因 (C/EBPδ、C/EBPβ、C/EBPα、PPARγ 及SREBP-1c) 表現量,及向下調控與三酸甘油酯合成之相關基因 (LPL、CD36、GLUT4、aP2、ACC1 及 FAS) 表現量,並減少脂肪細胞分泌抵抗素 (p<0.05)。以體內動物試驗模式評估,明道一號薑黃粉末 (MingDao No. 1 turmeric powder,MDTP)對於BALB/c小鼠不易形成體脂肪之效果。11週齡BALB/c雄性小鼠給予正常飼料(n=8)及高脂飼料(n=32)持續12週,分別作為控制組(normaldiet, CON)及誘導肥胖組。接著將肥胖之BALB/c雄性小鼠隨機分成 4 組(n=8),包括高脂組 (high-fat diet, HFD)、低劑量薑黃組(HFD + 1.25% MDTP, LCL)、中劑量薑黃組(HFD+2.5%MDTP, MCL)及高劑量薑黃組(HFD + 5% MDTP,HCL),持續餵食樣品12週後犧牲。實驗期間每週紀錄小鼠體重及飼料攝取量,犧牲後進行血清生化值分析和組織切片之判讀。結果顯示,LCL、MCL和HCL組之體重與HFD組相比,分別顯著降低13.27、12.42和9.91%;體脂肪率顯著降低2.05、2.03和2.09%;且血清中天門冬胺酸轉胺酶、胺基丙胺酸轉胺酶和三酸甘油酯含量也顯著降低 (p<0.05)。在肝組織切片結果顯示,HFD組脂肪肝分數(5.0?0.0)顯著高於CON組(1.9?0.6),而LCL、MCL和HCL組脂肪肝分數(3.3?1.0、2.9?0.6和 1.6?0.7)顯著低於HFD組(p<0.05),且呈現劑量效應關係。以 RT-PCR分析肝臟中基因表現量,結果發現LCL、MCL和HCL組之PPARα及CPT-1基因表現量與HFD 組相比顯著增加 (p<0.05)。綜合上述結果,tCur、aTur及MDTE可有效抑制3T3-L1前脂肪細胞分化及油滴的堆積;此外,餵食MDTP可有效地抑制BALB/c雄性小鼠體脂肪的生成。

English Abstract

Obesity is caused by an imbalance between energy intake and expenditure, which increases the size of adipocytes by conversion of extra energy to lipids and accumulates in adipose tissues. Obesity is associated with the variety of diseases such as diabetes, nonalcoholic fatty liver, dyslipidemia, hypertension and cardiovascular diseases. Preadipocyte differentiation is a major process to determine the number of mature adipocytes in the development of obesity. The aim of this study was to investigate the anti-adipogenesis effects of essential oil from Curcuma longa L. rhizomes (MingDao No. 1 turmeric essential oil, MDTE) in 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were exposed to the adipogenic medium followed by treated with curcumin (tCur), ar-turmerone (aTur) and MDTE during differentiation process. After 9 days treatment, the levels of lipid accumulation were determined by Oil Red O staining. The glycerol release and heparin-releasable LPL activity were used to evaluate the lipolysis and lipogenesis functions. RT-PCR (real time-polymerase chain reaction) analysis was used to estimate the gene expression of adipocytes differentiation and lipid metabolism. The results showed that 20 μM tCur, 1 μM aTur and 20 ~ 50 μg/mL MDTE treatments could significantly inhibit the lipid accumulation and MDTE increased the glycerol release by up-regulating lipolysis activity. The mRNA expressions of C/EBPδ, C/EBPβ, C/EBPα, PPARγ and SREBP-1c were reduced during preadipocytes differentiation, as well as LPL, CD36, GLUT4, aP2, ACC1 and FAS expressions were down-regulated in triglyceride synthesis pathway and resistin secretion was decreased by adipocytes. The preventive effect of fat accumulation by Curcuma longa L. (MingDao No. 1 turmeric powder, MDTP) was evaluated in vivo model. Eleven-week-old male BALB/c mice were fed normal diet as control group (n=8) and high-fat diet to induce obesity (n=32) for twelve weeks. Subsequently, obesity BALB/c mice were randomly divided into four groups (n=8), including high-fat diet (HFD), HFD plus 1.25% MDTP (LCL), HFD plus 2.5% MDTP (MCL) and HFD plus 5% MDTP (HCL) groups. All samples were fed for twelve weeks. Body weights and food intake of BALB/c mice were weekly recorded. After sacrifice, serum biochemical parameters and histopathological analysis were assayed. The results showed that administration of LCL, MCL and HCL significantly reduced body weights (13.27%, 12.42% and 9.91%), body fat ratio (2.05%, 2.03% and 2.09%), serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and triglyceride levels compared to the HFD group (p<0.05). Histopathological analysis revealed that the score of fatty liver in HFD group (5.0?0.0) was significantly higher than that of LCL, MCL and HCL groups (3.3?1.0, 2.9?0.6 and 1.6?0.7, respectively) (p<0.05). Real-time PCR analysis indicated that LCL, MCL and HCL increased the mRNA expression of PPARα and CPT-1 compared to the HFD group (p<0.05). In conclusion, tCur, aTur and MDTE can inhibit the differentiation and lipid accumulation in 3T3-L1 preadipocytes. Furthermore, oral administration of MDTP is beneficial for the suppression of fat accumulation in obese BALB/c mice.

Topic Category 農業暨自然資源學院 > 食品暨應用生物科技學系所
生物農學 > 生物科學
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