Title

臺灣產忍冬屬植物生物活性探索

Translated Titles

Investigation on the Bioactivities of Lonicera Plants Grown in Taiwan

DOI

10.6845/NCHU.2007.00053

Authors

楊鎮瑋

Key Words

裡白忍冬 ; 抗氧化 ; 抗發炎 ; 細胞毒性 ; 黃嘌呤氧化酶 ; Lonicera hypoglauca ; anti-oxidant ; anti-inflammatory ; cytotoxicity ; xanthine oxidase

PublicationName

中興大學森林學系所學位論文

Volume or Term/Year and Month of Publication

2007年

Academic Degree Category

碩士

Advisor

王升陽

Content Language

繁體中文

Chinese Abstract

忍冬屬植物為傳統藥用植物,民間記載具有清熱、解毒之療效。臺灣原生種忍冬屬植物計有阿里山忍冬、無梗忍冬、裡白忍冬、忍冬、川上氏忍冬、大花忍冬、玉山忍冬,及新店忍冬等八種。關於臺灣原生種忍冬屬植物生物活性之研究,至今仍不多見,為了開發此具發展潛力之藥用植物,本論文乃收集臺灣原生忍冬屬植物枝葉,並以中國產忍冬藤與金銀花為對照組,對其進行活性篩選工作。 忍冬屬植物抽出物在抗氧化活性方面,以裡白忍冬、忍冬及無梗忍冬抽出物之效果較佳。而抗發炎活性方面,則以裡白忍冬抽出物對抑制NO及PGE2之活性最好,且其乙酸乙酯可溶部在500 µg/ear濃度時,對抑制巴豆油誘導ICR小鼠耳朵發炎之抑制率為64.27%,相當於臨床藥物Indomethacin在300 µg/ear時之抑制率(56.02%)。此外,忍冬屬植物抽出物對Hep G2肝癌細胞毒性試驗之活性,以裡白忍冬抽出物之毒殺效果最佳。 於抑制黃嘌呤氧化酶活性試驗裡,忍冬屬植物抽出物中,以忍冬及裡白忍冬抽出物對黃嘌呤氧化酶之抑制活性最佳,而在裡白忍冬抽出物中分離出之Loniceraflavone,更表現極佳的抑制活性,其IC50值為0.85 µg/mL,效果與Allopurinol的0.41 µg/mL相當,然而做為臨床藥物的Allopurinol卻有造成腎病變的副作用。裡白忍冬抽出物乙酸乙酯可溶部在300 mg/kg B.W.劑量下,即對氧酸鉀誘導型高尿酸血症小鼠血清尿酸生成之模式具有抑制效果,其抑制率為31.91%。 本論文證實裡白忍冬及忍冬在抗氧化、抗發炎、細胞毒性及降尿酸等活性試驗方面,皆具有良好的效果,且已初步證實在活體試驗方面並不會對小鼠帶來副作用,若能繼續深入研究,將具有生物活性之各種成分分離並開發,相信可以在森林資源利用上更有效益並發展出具臨床療效之藥物或保健食品。

English Abstract

The Lonicera plants are used as the materials for traditional Chinese medicine since ancient time. There are eight Lonicera species, including Lonicera acuminate Wall., L. apodonta Ohwi., L. hypoglauca Miq., L. japonica Thunb., L. kawakamii (Hay.) Masam., L. macrantha (D. Don) Spreng., L. oiwakensis Hay., and L. shintenensis Hay., grown in Taiwan. The bioactivity study of Lonicera plants grown in Taiwan are rarely investigated until today. In order to explore and develop the potential application of Lonicera plants in medical application, their branches and leaves were collected for their bioactivities study in this thesis. As regard to the anti-oxidant activity of extracts from Lonicera plants, extracts from L. hypoglauca, L. japonica, and L. apodonta had greater activity. As for the anti-inflammatory activity of extracts from Lonicera plants, L. hypoglauca extract expressed the strongest activity for inhibition of NO and PGE2 formation. The ethyl acetate soluble fraction of the extract from L. hypoglauca had 64.27% of inhibition activity of croton oil-stimulated ICR mice at a dosage of 500 µg/ear. Its inhibition activity was equal to antiinflammation drug, indomethacin, at dosage of 300 µg/ear, which inhibited 56.02% inflammation. As regard to the xanthine oxidase inhibitory activity of Lonicera plants, extracts from L. japonica and L. hypoglauca shown the potent activity. A new compound, loniceraflavone, was isolated from the extracts of L. hypoglauca, which revealed the extremely great inhibition activity. The IC50 values of loniceraflavone for xanthine oxidase inhibitory activity was 0.85 µg/mL, which closed to allopurinol (IC50 values = 0.41 µg/mL). The ethyl acetate soluble fraction of L. hypoglauca could effectively inhibit generating uric acid of serum from the potassium oxonate-stimulated hyperuricemia mice at the dosage of 300 µg/kg (B.W.). Based on the results obtained in this study, the extracts from L. hypoglauca and L. japonica possessed good bioactivities, including anti-oxidant, anti-inflammatory, cytotoxicity, and reducing uric acid. Further, investigation in their phytocompounds will be obtained the good benefit for utilization of forestry resources and development of clinical treatment medicine or health food in the future.

Topic Category 農業暨自然資源學院 > 森林學系所
生物農學 > 森林
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Times Cited
  1. 李安哲(2011)。Active anti-hyperuricemia ingredients in Chrysanthemum indicum Linn. inhibits xanthine oxidase and reduces serum uric acid in rats.。清華大學生物科技研究所學位論文。2011。1-34。 
  2. 陶良榆(2011)。葉下珠之萃取成分可抑制黃嘌呤氧化酶以及降低大鼠血液中的尿酸。清華大學生物科技研究所學位論文。2011。1-43。 
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