Title

利用動物及細胞模式探討芸香素補充對代謝症候群指標及免疫調節的影響

Translated Titles

Effects of rutin supplementation on metabolic syndrome features and immunomodulation using murine and cell models

DOI

10.6845/NCHU.2011.00265

Authors

李萃芳

Key Words

代謝症候群 ; 芸香素 ; C57BL/6雄鼠 ; 3T3-L1脂肪細胞 ; 細胞激素 ; 抗發炎 ; 免疫調節 ; metabolic syndrome ; rutin ; male C57BL/6 mice ; 3T3-L1 adipocytes ; cytokines ; anti-inflammation ; immunomodulation

PublicationName

中興大學食品暨應用生物科技學系所學位論文

Volume or Term/Year and Month of Publication

2011年

Academic Degree Category

碩士

Advisor

林金源

Content Language

繁體中文

Chinese Abstract

代謝症候群的特徵包括腹部肥胖、高三酸甘油脂、高密度脂蛋白膽固醇過低、高血壓及高禁食血糖,代謝症候群是一系列心血管方面的危險因子,且易發展成為心血管疾病及第2型糖尿病。芸香素廣泛存在食物當中,包括:蕎麥、蘆筍、番茄及杏仁等。研究指出,芸香素具有重要生理活性,如:抗菌、抗發炎、抗氧化、抗病毒及抗癌細胞增生等功能,但芸香素補充對於代謝症候群及免疫調節作用仍不明確,因此本研究利用高脂飲食誘發動物產生代謝症候群,以探討芸香素之調節代謝症候群發炎反應與Th1/Th2免疫平衡的作用,且於細胞模式中,探討芸香素之調節前脂肪細胞發炎反應與分化的作用。動物實驗分成預防及治療模式,使用50%高脂飲食誘導C57BL/6雄鼠產生代謝症候群症狀,以測試芸香素的補充對於代謝症候群指標及免疫調節的影響,在細胞實驗中,則使用脂多醣刺激3T3-L1前脂肪細胞分泌促發炎細胞激素,以測試芸香素對發炎反應的調節及前脂肪細胞分化的影響。結果顯示,於體內模式中,補充芸香素於預防及治療模式下,皆可有效降低長期攝取高脂飲食小鼠之體重、副睪脂重、能量攝取、禁食血糖、三酸甘油酯、膽固醇及低密度脂蛋白膽固醇、脾臟發炎反應、Th1免疫傾向,且可改善肝臟與腎臟傷害及葡萄醣代謝異常現象;於體外模式中,芸香素可抑制3T3-L1前脂肪細胞分泌促發炎細胞激素及分化的情形。綜合以上結果顯示,芸香素於代謝症候群壓力下,可有效改善代謝症候群指標及調節發炎反應與Th1/Th2免疫平衡,而抑制脂肪細胞之發炎及分化為可能的途徑之一。

English Abstract

Metabolic syndrome features include abdominal obesity, hypertriglyceridaemia, low level in high-density lipoprotein cholesterol (HDL-c), hypertension and high level in fasting glucose. Metabolic syndrome is a cluster of cardiovascular risk factors that predispose an individual to develop cardiovascular diseases and type 2 diabetes. Rutin widely exists in various foods, including buckwheat, parsley, tomatoes and apricots. It has been shown to exert important biological activities, such as antimicrobial, anti-inflammatory, antioxidant, antiviral and antiulcerogenic effects. However, the effect of rutin supplementation on metabolic syndrome and immunomodulation is still not clear. Therefore, the study investigated effects of rutin on metabolic syndrome features, inflammation and Th1/Th2 immune balance using a high-fat diet fed murine model that induced metabolic syndrome, and the modulation of inflammation and differentiation of preadipocytes.The animal model was further devided into two models including preventive and curative models. A 50% high-fat diet was used to induce metabolic syndrome in male C57BL/6 mice. Lipopolysaccharide (LPS) was used to induce pro-inflammatory cytokines secreted by 3T3-L1 preadipocytes. The results showed that rutin supplementation markedly reduced body weight, epididymal fat, energy efficiency, fasting blood glucose, TG, cholesterol, LDL-c, spleen inflammation, Th1 immune response, as well as injury to the kidney and liver, and ameliorated the abnormal glucose metabolism in both preventive and curative models. In the in vitro model, rutin inhibited pro-inflammatory cytokines secreted by 3T3-L1 preadipocytes and differentiation of preadipocytes. The results suggest that rutin supplementation improved metabolic syndrome features, inflammation and Th1/Th2 balance under metabolic syndrome stress, possibly via inhibiting inflammation and differentiation of adipocytes.

Topic Category 農業暨自然資源學院 > 食品暨應用生物科技學系所
生物農學 > 生物科學
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