人類內皮細胞在不同濃度奈米金/銀-聚胺酯複合材料上之行為評估

Behavior of human endothelial cells on the nanocomposites of poly(ester)urethane and gold/silver nanoparticles of different concentrations.

10.6845/NCHU.2009.00767

何統作

二維電泳 ； 聚胺酯 ； 複合材料 ； 奈米金 ； 奈米銀 ； 細胞行為 ； 內皮細胞 ； 2-D ； polyurethane ； nanocomposite ； nano Au ； nano Ag ； cell behavior ； endothelial cell

中興大學化學工程學系所學位論文

2009年

碩士

徐善慧

繁體中文

本研究以二維電泳以及基因表現等方式，試圖找出內皮細胞受到不同濃度之奈米金-聚胺酯以及奈米銀-聚胺酯複合材料影響，調控開啟的訊息傳遞路徑，以找出不同的材料對於細胞之間的不同影響途徑。實驗結果發現，含43.5 ppm 奈米金的奈米金-聚胺酯，以及含30 ppm 奈米銀的奈米銀-聚胺酯，皆能明顯促進細胞生長以及Lamellipodia的形成，然而奈米金-聚胺酯似乎是透過Akt影響VCP進而促進eNOS活化，導致此項結果；而奈米銀-聚胺酯卻並非經由此種途徑來調控細胞。

This study is focused on how materials affect cell behavior and what signal transduction pathways are turned on when endothelial cells are grown on poly(ester)urethane (PSU)-Au/Ag nanocomposites. The activation of proteins and genes were analyzed by two-dimensional gel electrophoresis and reverse transcription PCR (RT-PCR). It was found that PSU-Au 43.5 ppm and PSU-Ag 30 ppm enhanced the proliferation and lamellipodia formation of endothelial cells. PSU-Au appeared to influence cell behavior by activation of valosin-containing protein (VCP) and the downstream molecule endothelial nitric oxide synthase (eNOS). On the other hand, PSU-Ag seemed to influence cell behavior via a different pathway.

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