Title

短鏈型硫代氨基甲酸類化合物對乙醯膽鹼酯酵 素、丁醯膽鹼酯酵素、膽固醇酯酵素、脂肪 酵素、青黴素酶及凝血酶抑制效果之研究

Translated Titles

Inhibition Effects of Acetylcholinesterase, Butyrylcholinesterase, Cholinesterase, Lipase, Penicillinase and Thrombin by Short-chain Thiocarbamates

Authors

王昱堯

Key Words

硫代氨基甲酸類化合物 ; 抑制劑 ; Thiocarbamates ; Inhibitor

PublicationName

中興大學化學系所學位論文

Volume or Term/Year and Month of Publication

2008年

Academic Degree Category

碩士

Advisor

林家立

Content Language

繁體中文

Chinese Abstract

本論文主要的研究是合成短鍊型硫代氨基甲酸類化合物做為乙醯膽鹼酯酵素 (AChE)、丁醯膽鹼酯酵素 (BChE)、膽固醇酯酵素 (CEase)、青黴素酶、脂肪酵素和凝血酶六種絲氨酸水解酵素,利用酵素催化三元組中絲氨酸所做的親核性攻擊反應機制,探討硫代氨基甲酸類化合物對於不同酵素抑制效果的研究,藉由停時驗析得知此類化合物皆屬類受質不可逆抑制劑。透過動力學實驗分析反應速率常數 (kc)、解離常數 (Ki)及總抑制常數(ki),可以得到化合物對於絲氨酸水解酵素的抑制結果。實驗中,由氨基甲酸類化合物、硫代氨基甲酸類化合物和市售藥物對乙醯膽鹼酯酵素等六種不同酵素之抑制常數,進一步可以比較抑制效果的差異。 實驗中,就本文所合成出的O,O'-propane-1,3-diyl bis(butylcarbamothioate) (化合物8)而言,對酵素的抑制效果由好到壞依序為,脂肪酵素、丁醯膽鹼酯酵素、膽固醇酯酵素、乙醯膽鹼酯酵素、青酶素酶和凝血酶;就化合物8和氨基甲酸類化合物比較,對乙醯膽鹼酯酵素、丁醯膽鹼酯酵素、膽固醇酯酵素和脂肪酵素而言,抑制效果則是化合物8較好;對青黴素酶和凝血酶而言,氨基甲酸類化合物則有較好的抑制效果。

Topic Category 基礎與應用科學 > 化學
理學院 > 化學系所
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Times Cited
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  2. 龔郁潔(2008)。定量結構分析短鏈型硫代氨基甲酸類化合物抑制青黴素酶及凝血酶。中興大學化學系所學位論文。2008。1-156。
  3. 王昶閔(2009)。硫代氨基甲酸類化合物構形分析與酵素抑制研究。中興大學化學系所學位論文。2009。1-188。