Title

熱致死乳酸桿菌促使老鼠脾臟細胞及經樹突細胞刺激之T細胞的免疫反應偏向Th 1

Translated Titles

Heat-killed cells of lactobacilli skew the immune response toward T helper 1 polarization in mouse splenocytes and dendritic cell-treated T cells

DOI

10.6845/NCHU.2007.00845

Authors

白欣怡

Key Words

乳酸桿菌 ; 脾臟細胞 ; 樹突細胞 ; 第一型輔助T細胞 ; 細胞激素 ; Lactobacillus ; splenocytes ; dendritic cells ; T helper 1 cells ; cytokines

PublicationName

中興大學食品暨應用生物科技學系所學位論文

Volume or Term/Year and Month of Publication

2007年

Academic Degree Category

碩士

Advisor

林美吟

Content Language

繁體中文

Chinese Abstract

乳酸菌為人類及哺乳類腸胃道中的共生細菌之一,已被認定為益生菌,對於維持宿主健康扮演著重要的角色,包括了其對於免疫功能的調節。目前大部分的研究皆比較著重於乳酸菌的活菌體 (viable cell) 及其細胞壁萃取物 (cell wall extract) 對於免疫調節作用的影響,而本實驗則主要是在於乳酸菌熱致死菌體的影響。我們藉由將三株熱致死乳酸菌L. acidophilus A2、L. gasseri A5和L. salivarius A6與脾臟細胞共同培養,來探討此三株熱致死乳酸桿菌對於免疫功能所產生的影響。另一方面,我們亦將三株熱致死乳酸菌L. acidophilus A2、L. gasseri A5和L. salivarius A6刺激樹突細胞後,再將經過刺激的樹突細胞與T細胞共同培養,觀察T細胞的免疫反應。由實驗結果顯示三株熱致死乳酸菌L. acidophilus A2、L. gasseri A5和L. salivarius A6,證實能刺激BALB/c小鼠的脾臟細胞增生和interleukin (IL)-10、IL-12 p70和interferon (IFN)-γ之分泌,但對transforming growth factor (TGF)-β無影響,使免疫反應偏向Th1型免疫反應。另外,三株熱致死乳酸菌亦能活化BALB/c小鼠的樹突細胞分泌大量的IL-12 p70,且經過刺激的樹突細胞能夠促使T細胞活化增生並顯著提升Th1型的細胞激素IFN-γ之分泌,但對IL-5, IL-13及TGF-β並無影響。以上結果顯示乳酸桿菌 (lactobacilli) 對於調節免疫機能及過敏反應扮演了重要的角色。

English Abstract

It is believed that probiotics play an important role for the health of the host including modulation of immune responses. Most studies have focused on the immunomodulatory effects of viable cells of lactic acid bacteria, however we investigated those of heat-killed cells of lactic acid bacteria in this study. We first observed the effects on immune functions via stimulating splenocytes with three heat-killed Lactobacillus strains. Furthermore, we also investigated the effect of mouse dendritic cells (DCs) treated with these heat-killed Lactobacillus strains on T cell responses. The results showed that these Lactobacillus strains were able to stimulate cell proliferation and interleukin (IL)-10, IL-12 p70 and interferon (IFN)-γ production, but not transforming growth factor (TGF)-β in splenocytes. In addition, these heat-killed Lactobacillus strains also stimulated high-level secretion of IL-12 p70 in DCs and switched T cells to T helper (Th) 1 immune responses, as evidenced by the elevated secretion of IFN-γ, but not IL-5, IL-13 and TGF-β. These results showed that lactobacilli play a potentially important role in modulating immune responses and allergic reactions.

Topic Category 農業暨自然資源學院 > 食品暨應用生物科技學系所
生物農學 > 生物科學
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