The effect of salinity on the skin mucous cell number and area of Barbronia weberi
巴蛭 ； 黏液細胞 ； 鹽度 ； Barbronia weberi ； mucous cells ； salinity
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The discharge of domestic wastewater, industrial effluents and livestock wastewater into river has had a great impact on the water quality of the river. It has been a common practice to use ground water for irrigation in the farm near the coastal areas, and caused land salinization in these regions in Taiwan. Barbronia weberi was identified as a biological indicator species for low and medium pollution water in Han River in central Taiwan. The mean number of pear shaped secretory cells counted in the samples of B. weberi collected from the river water with medium pollution level is greater than the samples collected from the river water with low pollution level. The environmental factor to cause the physiological mechanism of the cytological outcome is difficult to evaluate due to the dynamic changes of the chemical composition in the water. In order to find the physiological adaptation of B. weberi to a single environmental factor such as salinity, we collected wild B. weberi and bred a testing population in laboratory. The study of mucous cell and its secretion from the offspring of B. weberi exposed to various salinity levels of water was conducted in a laboratory controlled environment. The main composition of mucus from B. weberi are polysaccharide and protein. The lethal concentration 50% (LC50) of B. weberi was calculated from the TRAP program to be 33% of sea water or 10.25 g salt/kg in this study. The mean number and area of round mucous cells from the paraffin section of B. weberi were significantly greater in the 30% sea water treatment than those found in 12.5%, 25% sea water and control treatments. However, there is no significant difference of mean number and area of pear shaped secretory mucous cells among the salinity treatments. The results of this study indicated that round mucous cells secrete more mucus to help the B. weberi adapt to environment with low salinity water. The pear shaped secretory mucous cells secrete acid mucus to control the bacteria infection, therefore, there is no response of the pear shaped mucous cell in terms of mean number and area to the salinity change in water. Two different functions of the mucous cell of B. weberi may account for the adaptation of B. weberi in the low and medium levels of pollution water in the river. The adaptation trait may help B. weberi to be a successful exotic in Europe and United States.
生物農學 > 生物科學