Title

細菌纖維素生產菌之篩選及其最適培養條件之研究

Translated Titles

Screening of bacterial cellulose producer and optimization of its culture conditions

Authors

沈玫彣

Key Words

細菌纖維素 ; 醋酸菌 ; 反應曲面法 ; Bacterial cellulose (BC) ; Acetobacter strains ; response surface methodology (RSM)

PublicationName

中興大學食品暨應用生物科技學系所學位論文

Volume or Term/Year and Month of Publication

2007年

Academic Degree Category

碩士

Advisor

黃文哲

Content Language

繁體中文

Chinese Abstract

本實驗初步篩選十二株醋酸菌中,以Gluconacetobacter sp. C07有較佳之細菌纖維素產量及完整之纖維素片,因此選擇以Gluconacetobacter sp. C07為發酵菌種,探討其於SH培養基中進行生產細菌纖維素之最適發酵條件。 探討發酵條件方面,以靜置培養之活化菌種方式及選擇以10 %(v/v)之接種量探討溫度及pH值對細菌纖維素產量之影響,由結果可發現以30℃之培養溫度及較高的初始基質pH值(pH = 6.0)下可得較佳之細菌纖維素產量。 探討基質組成對細菌纖維素產量影響方面,挑選蔗糖(sucrose)及酵母萃取物(yeast extract)兩因子以反應曲面法探討其濃度對纖維素生成之影響,得到最適發酵條件為Gluconacetobacter sp. C07於初始基質pH 6.0之添加有5.87%蔗糖及1.81%酵母萃取物之SH培養基中,於30℃下靜置培養12天後可得最大細菌纖維素產量(1.82公分),經實際測試可得1.81公分。 物性及機械測試方面,以本試驗最適發酵條件所得之纖維素片與市售之纖維素片比較可發現本試驗最適發酵條件所得之纖維素片之離水性、保水力、水含量、破碎度、附著度較市售纖維素片為高;硬度、彈性、凝聚度、凝膠強度、咀嚼性、彈回力則較市售之纖維素片為低。

English Abstract

Preliminary screening twelve Acetobacter strains in this study, Gluconacetobacter sp. C07 had a better bacterial cellulose(BC) yield and cellulose-pellicle integrity than others. Therefore we chose Gluconacetobacter sp. C07 as the fermentative bacterium and determined the optimal fermentation conditions for the bacterial cellulose production in SH medium . To optimalize the fermentation conditions, we used bacterial culture that activied by static incubation and a 10 % (v/v) inoculum size to study the effect of temperature and pH on BC yield. Results indicated that 30℃ and the higher initial substrate pH (pH = 6.0) could result in a better BC yield. To investigate the effect of substrate compositon on BC yield, the concentrations of two factors (sucrose and yeast extract) were statistically estimated by Response Surface Methodology (RSM). The optimization fermentation condition for the largest BC yield (1.82 cm) by Gluconacetobacter sp. C07 grown in the SH medium is that added sucrose and yeast extract ( 5.87 and 1.81 g/100ml respectively) in SH medium and initial substrate pH 6.0 at 30℃ for 12 days under static condition. The practical tests by using optimal condition can produce the BC with the similar yield (1.81 cm). For the physical and mechanical properties, the cellulose-pellicle from optimization fermentation codition in this study was compared with cellulose-pellicle from the market. Results showed that the cellulose-pellicle in this study was higher in syneresis, water-holding capacity, water content, fracturability and adhesiveness than that from market, and hardness, springiness, cohesiveness, gumminess, cheesiness, resilience were lower than the cellulose-pellicle from the market.

Topic Category 農業暨自然資源學院 > 食品暨應用生物科技學系所
生物農學 > 生物科學
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