Title

無患子木材及果實萃取物之抗菌活性與植物相剋作用

Translated Titles

Antifungal and Allelopathic Activities of Extracts from the Wood and Fruits of Sapindus mukorossi

DOI

10.6845/NCHU.2006.00791

Authors

嚴振賓

Key Words

無患子 ; 抗真菌活性 ; 相剋作用 ; Sapindus mukorossi ; antifungal activity ; allelopathy

PublicationName

中興大學森林學系所學位論文

Volume or Term/Year and Month of Publication

2006年

Academic Degree Category

碩士

Advisor

陳載永

Content Language

繁體中文

Chinese Abstract

本研究之主要目的為分析無患子(Sapindus mukorossi)木材與果實抽出成分之抗真菌活性以及植物相剋作用。抑菌活性試驗結果顯示,無患子木材抽出物以正丁醇(n-BuOH)分離部對青黴菌(Penicillium sp.)具有抑制效果,無患子果實抽出物之乙酸乙酯(EtOAc)和正丁醇分離部對青黴菌、麴菌(Aspergillus sp.)及黑黴菌(Aspergillus niger)具有抑制效果,其IC50值均小於1,000 μg/mL。處理竹材耐腐朽能力顯示,無患子抽出物處理之竹材於試驗第28-56天時其所造成之重量損失率最大者僅為1.2%。至於植物相剋作用活性方面,由小白菜種子發芽抑制試驗結果得知,無患子木材與果實抽出物各分離部皆無法抑制種子的發芽,但二者之正丁醇分離部均可延緩種子發芽作用36 h。此外,木材抽出物之水分離部則具有抑制胚根生長的作用,經濃度1,000 μg/mL處理72 h之小白菜,胚根與胚莖長比值為0.69,且其胚根乾重僅為對照組的20.6%,但無患子抽出物對正常生長7天後之小白菜並無顯著抑制生長作用。

English Abstract

The effect of wood and fruit extracts from Sapindus mukorossi on the antifungal and allelopathic activities were evaluated in this study. After serial partition, the n-BuOH fraction of wood extract shows the best antifungal activity against Penicillium sp. among all soluble fractions. As for the fruit extract, the EtOAc and n-BuOH fractions also exhibit a good antifungal activity. The IC50 values of both fractions were less than 1000 μg/mL against 3 test fungi, Penicillium sp., Aspergillus sp., and Aspergillus niger. The results of bamboo decay test show that the extracts of Sapindus mukorossi cause the weight loss of bamboo was only 1.2% in period of 28-56 days. On the other hand, the crude extracts of Sapindus mukorossi (wood and fruit) could not inhibit the seed germination on Pak-choi, but their n-BuOH fractions could retard the germination for 36 h. Additionally, the water fraction from wood extract can inhibit the root growth on Pak-choi. At 1000 μg/mL, after 72 h, the length ratio of root to shoot was 0.69, and the dry weight of Pak-choi root was only the 20.6% of control. But the extracts of Sapindus mukorossi have no influence on the growth of Pak-choi with normal growth 7 days.

Topic Category 農業暨自然資源學院 > 森林學系所
生物農學 > 森林
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Times Cited
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