Title

蓬莪蒁及春鬱金對3T3-L1前脂肪細胞及高脂飲食誘導肥胖之C57BL/6J小鼠不易形成體脂肪之探討

Translated Titles

Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice

Authors

王啟憲

Key Words

蓬莪蒁 ; 春鬱金 ; 肥胖 ; 3T3-L1 ; 高脂飲食 ; C57BL/6J小鼠 ; Curcuma phaeocaulis ; Curcuma aromatica ; obesity ; 3T3-L1 ; high-fat diet ; C57BL/6J mice

PublicationName

中興大學食品暨應用生物科技學系所學位論文

Volume or Term/Year and Month of Publication

2015年

Academic Degree Category

碩士

Advisor

蔣慎思

Content Language

繁體中文

Chinese Abstract

肥胖為長期攝食過多的三酸甘油酯並囤積於脂肪組織中,造成體脂肪過高而影響健康的一種狀態。本研究以細胞試驗及動物試驗探討蓬莪蒁 (Curcuma phaeocaulis) 及春鬱金 (Curcuma aromatica) 不易形成體脂肪之功效。在細胞試驗中,以蓬莪蒁之精油 (Curcuma phaeocaulis essential oil, CPO) 及春鬱金之精油 (Curcuma aromatica essential oil, CAO) 與春鬱金乙醇萃取物 (Curcuma aromatica ethanol extract, CAE) 評估其抑制 3T3-L1 前脂肪細胞分化及脂解之效果。結果顯示 50-100 g/ml CPO 及 500g/ml CAE可顯著抑制脂質的堆積。利用即時聚合酶連鎖反應 (real-time polymerase chain reaction, RT-PCR) 分析前脂肪細胞分化、脂質合成及脂質分解相關基因表現量,結果顯示,100 g/ml CPO 能減少脂質合成相關 (acetyl-CoA carboxylase, ACC) 基因表現量 (53.4±12.4%) 並增加脂解相關基因 (carnitine palmitoyltransferase I, CPT-1) 表現量 (91.0±52.2%)。500g/ml CAE 降低前脂肪細胞分化期間之 (peroxisome proliferator-actived receptor gamma, PPAR (54.5±14.5%) 及脂質合成 ACC (67.4±8.8%) 表現量並增加脂質分解路徑基因 CPT-1 (168.6±57.7%) 表現量。在動物模式中,利用蓬莪蒁粉末 (Curcuma phaeocaulis powder, CPP) 及春鬱金粉末 (Curcuma aromatica powder, CAP) 為餵食樣品。以八週齡 C57BL/6J 雄性小鼠給予正常飼料 (n=10) 作為控制組 (CON) 及高脂飼料 (n=70) 為誘導肥胖組持續10週。將成功誘導肥胖之小鼠隨機分成7組 (n=10),包括高脂組 (HFD),低、中、高劑量蓬莪蒁組 (LCP、MCP 及HCP) 及低、中、高劑量春鬱金組 (LCA、MCA 及HCA),餵食 10 週後犧牲,犧牲後進行血清生化值分析和組織切片之判讀。結果顯示,蓬莪蒁及春鬱金組之小鼠體重與 HFD 組相比,顯著降低 19.4-28.8 % 與 14.4-36.5 %,且具有劑量效應 (p<0.05);體脂肪比率降低 1.6-3.1 % 與0.4-4.0 %;各組副睪脂肪細胞平均半徑皆比 HFD 小,且血清中 aspartate aminotransferse, AST、alanine aminotransferase ALT 及 leptin含量也顯著降低 (p<0.05)。在肝臟組織切片結果顯示,HFD 組脂肪肝分數為 4.5±0.8,而蓬莪蒁及春鬱金組之脂肪肝分數較 HFD 組低,分別介於2.1-3.1與1.3-3.8,且春鬱金組具有劑量效應。綜合上述結果,蓬莪蒁精油及春鬱金乙醇萃取物可有效降低 ACC 與提升 CPT 基因表現,達到抑制 3T3-L1 前脂肪細胞油滴的堆積;此外動物試驗結果亦說明餵食蓬莪蒁及春鬱金粉末具有降低體重及體脂率,減少副睪脂質堆積,改善高脂飲食所誘導脂肪肝,具有不易形成體脂肪的潛力。

English Abstract

Obesity is caused by extra energy intake, which increase the size of adipocytes and make body unhealthy. The aim of this study was to investigate the preventive effects of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica in vitro and in vivo. The in vitro results indicated that 50-100 g/ml CPO and 500g/ml CAE can significantly inhibit the lipid accumulation by oil O staining. RT-PCR (real-time polymerase chain reaction) analysis was used to estimate the gene expression of adipocytes differentiation and lipid metabolism. The results showed that 100 g/ml CPO treatment can decrease the expression of adipogenesis-related gene ACC (53.4±12.4%) and increase the expression of lipolysis-related gene CPT-1 (91.0±52.2%). 500g/ml CAE treatment can decrease the expressions of preadipocytes differentiation-related gene PPAR(54.5±14.5%) and ACC (67.4±8.8%), but increase the expression of CPT-1 (168.6±57.7%). The preventive effect of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica powder (CPP and CAP) was evaluated in vivo. Eight-week-old male C57BL/6J mice were fed normal diet as control group (n=10) and high-fat diet to induce obesity (n=70) as obese group for ten weeks. Subsequently, obese group mice were randomly divided into seven groups (n=10), including high-fat diet group (HFD); low, medium and high-doses of Curcuma phaeocaulis group (LCP、MCP and HCP) and low, medium and high-doses of Curcuma aromatica group (LCA、MCA and HCA). These mice were fed for eight weeks and sacrificed, the serum biochemical parameters and histopathological analysis were assayed. The results showed that administration of CA and CP powders reduced the ratio of body weights range from 19.4-28.8 % and 14.4-36.5 % (p<0.05), and body fat ratio from 1.6-3.1 % and 0.4-4.0 %. Moreover, the size of adipocytes was smaller in all treatment groups than HFD group. The serum parameters of AST, ALT and leptin levels in treatment groups were reduced compared to HFD group. Histopathological analysis revealed that LCP, MCP, HCP, LCA, MCA and HCA decreased the pathological change of fatty infiltration in liver from score 4.5±0.8 (HFD group) to score 2.6±1.6, 3.1±1.2, 2.1±1.3, 3.8±1.0, 3.4±1.4 and 1.3±1.3, respectively. In conclusion, essential oil of Curcuma phaeocaulis and ethanol extract of Curcuma aromatica can inhibit lipid accumulation in 3T3-L1 preadipocytes by decreasing the expression of ACC and increasing the expression of CPT gene. Furthermore, oral administration of CP and CA powder can reduce the body weight, body fat ratio, fat accumulation in epididymal fat and severity of fatty liver in the mice.

Topic Category 農業暨自然資源學院 > 食品暨應用生物科技學系所
生物農學 > 生物科學
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