開發多孔性聚乳酸微粒作為軟組織填補劑之應用

Development of porous polylactic acid for the soft tissue filling applications

黃曼婷(Man-Ting Huang)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/化學工程研究所/碩士(2013年)

https://doi.org/10.6841/NTUT.2013.00095

摘要

本實驗研究內容為開發多孔性聚乳酸作為真皮刺激物，主要機制是藉由注入的生醫材料觸發發炎反應，使纖維母細胞靠近，進而分泌膠原蛋白，達到真皮增生之目的。除了可以達到填補效果，並可以延長填補時效。
因為其生物可分解性、生物可吸收性及生物相容性的性質，已經被廣泛的運用在生物醫學上。利用聚乳酸，開發具有多孔性微粒之生醫材料，同時建立多孔性聚乳酸微粒製程，完成性質測定及找出最佳條件、材料對細胞毒性檢測等。聚乳酸搭配凝膠形成注射型軟組織填補劑注入所需人體填補部位，如凹陷部位。
目前市面上之真皮刺激分子，常見的有氫氧基磷灰石及聚乳酸等，現行缺點問題在於材料製程方面，則是顆粒大小不一致，表面形貌不規則且材料於填補劑內分散性不佳，因而導致真皮刺激反應不均勻，局部有過量反應，臨床現象 像是丘疹及肉芽組織腫大。為提升應用性，透過本製程所得到多孔性聚乳酸微粒開發作成填補劑與已上市的品牌-Sculptra做比較，例如：外觀、黏彈性、分散性等。最後，將脂肪幹細胞和所選定多孔性聚乳酸填補劑相混，檢測生物相容性。
因此本實驗目的是，開發大小形貌均勻之多孔性聚乳酸微粒改善現行真皮刺激不均填補效果，並且優化填補劑配方，達自然柔軟效果。

關鍵字

聚乳酸 ； 生物可分解性 ； 生物相容性 ； 填充劑 ； 脂肪幹細胞

並列摘要

The experimental research for the development of porous polylactic acid as a dermal stimulant, the main mechanism is implanted biomaterials by inducing inflammation, so that fibroblasts close, and then secrete collagen, reaching the dermis proliferation purposes. In addition to filling effect can be achieved, and can be extended to fill the prescription.Because of its biodegradable, bioabsorbable and biocompatible nature, has been widely used in biomaterial applications. Use of polylactic acid, the development of
porous particles, while establishing a porous polylactic acid particle processes, complete determination of the nature and identify the best conditions, the material on cytotoxicity detection. Polylactic acid with a gel-forming injectable soft tissue filling agent is injected into the body to fill the necessary parts, such as fold part.
Currently on the market, often with hydroxyapatite and polylactic acid, etc. The problem is that the existing shortcomings material fabrication process, it is different
particle size, surface morphology and the material in the filling material irregularities within the dispersion is not well, resulting in uneven dermal irritation, locally
excessive reaction, clinical phenomena such as pimples and granulation tissue swelling. To enhance the application, obtained through the process of polylactic acid particles porous filling material made with the development of the brand-Sculptra listed for comparison, for example: the appearance, viscoelasticity, dispersion and so on. Finally, the selected adipose stem cells and polylactide porous filling material mixed, testing biocompatibility.
Therefore, the purpose of the experiment is to develop a uniform size of the porous morphology of polylactic acid particles to improve the existing dermal stimulation uneven fill effects, and optimizing fill formulations, the effect of the natural softness.

並列關鍵字

Polylactic acid ； Biodegradable ； Biocompatible ； Filler ； Adipose-Derived Stem Cell

參考文獻

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