製備多孔性聚乳酸微粒於軟組織填補劑之開發

Preparation of porous polylactic acid particles for the development of soft tissue fillers

吳書璇(Shu-Hsuan Wu)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/生物科技研究所/碩士(2014年)

https://doi.org/10.6841/NTUT.2014.00754

摘要

因外力或是疾病、造成皮下軟組織的缺陷，不僅影響患者正常皮膚功能、軟組織外觀缺陷，也影響心理層面受創，因此組織缺陷影響層面之深遠，皮下軟組織修復的問題需要我們好好的治療改善。刺激性真皮增生填補劑為誘導自體膠原蛋白生成且具中長效填補效果，因而成為臨床填補不錯選擇。然而目前真皮刺激填補劑材料因顆粒大小不一致，形貌不規則，導致丘疹和肉芽組織腫大等臨床問題仍需被克服。本研究目的製備多孔性聚乳酸微粒做為軟組織填補劑，利用雙乳化法製備規則之微粒，以改善刺激性填補劑填補效果不均之問題。

本研究透過調整雙乳化法製程中參數，製備出聚乳酸微粒，利用體外細胞培養模式篩選出最具真皮刺激潛力之微粒，篩選微粒以調整最佳化填補劑配本實驗調控轉速製造不同大小微粒，借由增加聚乳酸與聚乙烯醇體積比，改善產率及多孔性。在細胞實驗中，小微粒培養使細胞生長活性佳，多孔性微粒能使降解膠原蛋白基因MMP1表現量減少。

關鍵字

聚乳酸 ； 雙乳化法 ； 填補劑

並列摘要

The external force or disease made soft tissue defect, it not only lost normal function of the skin, but also affected the psychological trauma. Therefore we need good treatment to improve organizational defects of subcutaneous soft tissue repair problems. The stimulatory fillers induced autologous collagen production and had a long-term fill effects, Thus they became a good choice of clinically fill . However, there are still problems of the stimulatory fillers such as particle size, morphology irregular lead to granulation tissue swelling and other clinical problems need to be overcome. Therefore the purpose of the study was prepared porous polylactic acid particles to improve the uneven filling. The study was prepared many kinds of porous polylactic acid particles in a variety of double emulsion parameters and used the biocompatible porous polylactic acid particles, gene expression, and to optimize the dispersion well filler.
We used speed regulation and different volume ratio of polylactic acid and polyvinyl alcohol to make different sizes particles and increased the yield. In cell experiments, small polylactic acid particles had good activity and porous polylactic acid particles can reduce the degradation of collagen gene MMP1 expression.

並列關鍵字

Polylactic acid ； Double emulsion solvent evaporation method ； Filler

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