脈衝式射頻療法(PRF)應用在治療(大鼠) 背根神經痛的基因表現量的研究

Gene expression of Pulsed Radiofrequency (PRF) Applied to Dorsal Root Ganglion (DRG) on Neuropathic Pain in Rats

羅君維(Chun-Wei Lo)

指導教授 ： 李元綺

共同指導教授 ： 溫永銳

臺北醫學大學/醫學科技學院/醫學資訊研究所/碩士(2012年)

https://doi.org/10.6831/TMU.2012.00007

摘要

臨床上脈衝式射頻療法(PRF)可以治療不同的疼痛症狀，但是其分子作用機轉還有許多未知。本篇從生物晶片來觀察相關基因的表達，實驗設計先將實驗大鼠施以脊神經結紮手術(SNL)，其目的是為了製造機械性疼痛。實驗分為實驗組(SNL組)-有進行SNL手術，與對照組(Sham組)。兩組皆進行三種不同時間的PRF刺激：沒刺激，刺激5分鐘和10分鐘。每一個實驗組皆重複三次，總共得到18個樣本，18個晶片數據。相互比較每一種情況下基因表現量的差異，可分類為SNL effect, side effect, PRF effect, time effect等，闡明在施予PRF治療時，體內所產生生理對疼痛的產生的免疫反應，以及所引起基因調控，細胞信號轉變等機制。

關鍵字

脈衝式射頻療法；基因表現微陣列生物晶片；神經痛

並列摘要

The mechanism and efficacy of pulsed radiofrequency (PRF) are largely unknown. In this study, we investigated the gene expression of analgesic effect of PRF on neuropathic pain in a spinal nerve ligation (SNL) rat model. Both sham and SNL group were stimulation of 2.5V sine-wave pulses for non-treat, 5 minutes and 10 minutes. To avoid biological variation, all samples are in triplicate with a total 18 samples and 18 gene chips using Affymetrix Gene Array Rat 1.0 st array. Samples are treated with spinal nerve ligation (SNL) and sham surgery was done in parallel. They were treated with 0, 5, and 10 minutes PRF, respectively. The gene expression data are analyzed according to their specific characteristics, such as SNL effect, PRF(sham) effect, PRF(SNL) effect：, time effect. In this rat model, it is expected that we could understand more what genes related to pain are responsive to PRF and what genes are subject to be developed more different treatment in the future.

並列關鍵字

Pulsed-radiofrequency (PRF)； gene expression； neuropathic pain

參考文獻

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