利用低溫電漿處理鈦金屬表面之蛋白質固定

Protein Grafting on the Titanium Surfaces Using Glow Discharge

陳錦煜(J-Y CHEN)

指導教授 ： 林哲堂 ； 李勝揚

臺北醫學大學/口腔學院/牙醫學系碩博士班/碩士(2002年)

摘要

人工牙根植入後仍需三至六個月頗為漫長的癒合時間，且其骨整合程度經常不如預期，因此進行其鈦金屬之表面處理，以有效縮短骨癒合時間並強化骨整合，就變得格外重要。本研究利用低溫電漿來活化鈦金屬表面以連接第一型膠原蛋白。鈦金屬表面先以丙烯胺電漿處理，使附著上胺基(-NH2)，再分別以不同濃度的交鏈劑BS3及戊二醛連接固定第一型膠原蛋白。而後以電子顯微鏡(SEM)觀察樣本的表面，以電子能譜儀(XPS)與顯微傅立葉轉換紅外線光譜儀(Microscopic-FTIR)作定性分析，並以元素分析儀(SEM-EDS)作定性分析與半定量分析，檢測蛋白質在不同濃度和種類的交鏈劑處理下在鈦金屬表面連接的情況。SEM結果可見，實驗組的鈦金屬上有球型或海綿狀類似膠原蛋白之披覆。FTIR則檢測出有C=O, OH, 醯胺Ⅰ鍵和醯胺Ⅱ鍵，可能為膠原蛋白本身結構中的醯胺鍵結，或是電漿處理後鈦金屬表面的醯胺鍵結。而XPS分析圖中也顯示有C、C=O、C-C、COOR、N、O等膠原蛋白的結構存在；並由SEM-EDS數據顯示，第一型膠原蛋白的量會隨著戊二醛或BS3濃度的升高而增加。經電漿與交鏈劑BS3或戊二醛的處理並固定第一型膠原蛋白後之實驗組類成骨細胞(MG 63)培養4小時後觀察，和對照組相比較，可見骨細胞伸出無數觸角延伸向有膠原蛋白纖維處，培養24小時後，可見骨細胞型態變為扁平並平鋪且深入具有膠原蛋白纖維處。本研究結果顯示，使用電漿處理技術可達成鈦金屬表面胺基化，加上交鏈劑BS3或戊二醛的處理後，可能使鈦金屬表面接上第一型膠原蛋白，且會隨著交鏈劑的濃度升高而增加，並可加速骨細胞生長。

關鍵字

鈦金屬 ； 低溫電漿 ； 交鏈劑 ； 第一型膠原蛋白

並列摘要

One of the disadvantages for the dental implants is the six-month healing time. To shorten the healing time of osseointegration is an important issue for the dental implant development. Glow Discharge is a surface treatment to activate surface characters using gas plasma. Collagen is a key factor during the healing procedure and bone structuring. In this study, collagen connected titanium plates were evaluated in vitro. The titanium plates were treated by glow discharge with allylamine. Glow discharged titanium plates were connected with TypeⅠcollagen by amine cross-linking agents BS3 and Glutaraldehyde. The collagen-coated titanium plates were evaluated by their surface condition with scanning electron microscope-energy dispersive spectrometer (SEM-EDS) and microscopic FTIR and X-ray photoemission spectroscopy (XPS). There were collagen component elements C, S, N elements detected on the titanium surfaces. And collagen structural bonds C-N, C=O, C=C bonding were detected on the titanium surfaces by XPS and microscopic FTIR. The data proved that collagen were successful grafted on the titanium surfaces. The counts of collagen were increased with the increase of concentrations of BS3 or Glutaraldehyde detected on the titanium surfaces by SEM-EDS. Osteoblast-like cells (MG 63) were cultured on the treated titanium plates for 4 or 24 hours. The result showed the osteoblast-like cells spread radically after 4 hours, and they were almost flat after 24 hours. Therefore, collagens were successful grafted on the titanium plates by glow discharge technology. This method could improve the growth and spread of human osteoblasts.

並列關鍵字

Titanium ； glow discharge ； cross-linking agent ； TypeⅠcollagen

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