- 學位論文
探討環境中生產聚羥基烷酸之微生物
Characterization of polyhydroxyalkanoates- producing bacteria isolated from environment
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摘要
聚羥基烷酸 (polyhydroxyalkanoates；PHAs) 是一種微生物合成的生物可分解高分子，某些細菌當處於碳源過剩且缺乏某一類養分(例如：氮、磷或硫等)的環境時，就會合成PHAs並累積於菌體內。聚羥基丁酸 (polyhydroxybutyrate；PHB)為環境中微生物累積最常見的PHAs種類。環境中遍佈著各式各樣具有累積PHAs的微生物，微生物與其所合成之PHAs的多樣性一直是一個非常受到重視的研究主題，腸內菌 (enteric bacteria)為環境中分佈非常廣泛的細菌，然而長久以來，未有文獻針對腸內菌是否具有生產PHAs的能力加以探討。許多已完成基因體定序之腸內菌微生物 (如E. coli與 Salmonella等)亦未發現其帶有PHAs合成的相關基因。這些用來定序的菌株大都是臨床菌株或是實驗室保存之菌株。 在篩選與鑑定環境微生物的工作時，我們發現一些具有累積PHB的革蘭氏陰性菌，其特性以及16S rDNA序列顯示與腸內菌科的微生物接近，本實驗的主要目的乃在於探討是否在環境中存在一些具有合成這類高分子的微生物族群。
並列摘要
PHAs (polyhydroxyalkanoates) is biodegradability polymers which is synthesized by microorganisms, some bacteria will synthesize PHAs and accumulated in vivo when the environment have excess carbon source and lack of some nutrients (ex: nitrogen, phosphate or sulfate). PHB (polyhydroxybutyrate) is the most common type of PHAs which is accumulated by microorganisms in the environment. The enteric bacteria can be commonly found in many environments; however, there is no report on their ability for PHAs production. From complete genome sequences of some enteric bacteria (e.g. E. coli and Salmonella), there was also no evidence of genes related to PHS synthesis in their chromosomes. Most of these sequenced strains are from culture collection and clinical samples. During the process of screening PHA-producing microorganisms in the environment, we have isolated some Gram negative bacteria that can accumulate PHB and presumably belong to enteric bacteria according to their 16S rDNA sequences analysis. In this study, we examined if these environmental enteric bacteria can accumulate PHB.
參考文獻
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