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  • 學位論文

使用乙二醇當萃取液搭配中空纖維動態三液相微萃取/高效液相層析儀偵測環境水樣中的芳香胺類化合物

Use ethylene glycol as extraction solvent for hollow fiber dynamic liquid-liquid-liquid microextraction to determine aromatic amines by HPLC in water samples

指導教授 : 黃賢達
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摘要


近年來,綠色化學的趨勢逐漸成為全球所關注,如何應用少量的化學藥品達到研究成果,更是被受關注。本文採用動態三液相微萃取(Dynamic-LLLME)強調快速、簡便、低成本及低污染,作為環境水樣中七種苯胺類(aniline;benzidine;o-Toluidine;4-nitroaniline;4-chloroaniline;4-bromoaniline;3,4-dichloroaniline)污染物的濃縮萃取,並使用配合高效液相層析儀/二極體陣列偵測器(HPLC/PDA)進行分離與分析。 本研究的苯胺類化合物已被研究證實有致癌的危險性,其中聯苯胺(Benzidine)被美國國家環境保護署(EPA)列為第一控管污染物。為達低偵測極限,在前濃縮方面探討使用多元醇當萃取液,和添加不同種類的酸研究其對分析物萃取效果之影響,而其他各項可能影響萃取效率的變因也進行詳細探討。並實際應用本方法於環境水樣(寶山水庫水、濁水溪、淡水河)。 於最佳實驗條件下,方法偵測極限(MDLs)在0.1~2.0 μg/L;線性相關係數(r2)在線性濃度範圍內均大於0.9989;線性濃度因不同化合物而異,大致落在0.5~500 μg/L內;精密度方面,相對標準偏差RSD (%),在試劑水落在3.3~8.4%之間,而在環境水樣落在3.6~9.2%之間。對於準確度的測試,作三種環境水樣的相對添加回收率分別為93.6 %~102.5 %、86.5 %~105.2 %及85.5 %~104.8 %,顯示本分析方法不易受到基質的干擾,可成功應用於環境水樣之分析芳香胺類化合物。

並列摘要


In recent years, green chemistry has become the main point in global chemistry research. In this research ,we use dynamic-LLLME which stress fast、 simple、low-cost and low-pollution technique to concentrate seven aromatic amines(aniline;benzidine;o-Toluidine;4-nitroaniline;4-chloroaniline;4-bromoaniline;3,4-dichloroaniline) in environmental water then separate and determine by high performance liquid chromatography(HPLC)and Photo Diode Array (PDA) detector. Aromatic amines are proven to be carcinogenic , especially benzidine has been regulated as priority pollutant by U.S. Environmental Protection Agency (EPA) . In order to reach low detection limits, we use polyhydric alcohols as extraction solvent and find that different anion from acid affect extraction . This work also makes a detailed research about other factors that mainly influence extraction results, and applies the proposed method to reservoir water 、stream water and river water. Under the optimum experiment conditions, method of detection limits(MDLs) range from 0.1~2.0 μg/L; Good linearity of analytes is achieved in the range of 0.5~500 μg/L with coefficients of determination, r2 > 0.9989; Good reproducibilities of extraction performance are obtained with RSD (%)3.3~8.4 in test water and 3.6~9.2 in environmental water .The spiked relative recoveries of the three real samples are in the range of 93.6 %~102.5 %、86.5 %~105.2 % and 85.5 %~104.8 %, respectively. This reveals the proposed method can successfully apply to the analysis of aromatic amine from environmental water samples.

參考文獻


究”國立臺灣大學化學研究所碩士論文(1999)
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